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A degradable and highly porous polyurethane scaffolds for bone tissue engineering

Monika Bil ¹, Krzysztof J. Kurzydlowski ¹, Joanna Ryszkowska ¹, Piotr Woźniak ², Małgorzata Lewandowska-Szumieł ²

1. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland

Abstract

Scaffold-based tissue engineering requires for transplanted or host cells a biodegradable matrix, which provides a specific environment for tissue development. Efficiency of tissue regeneration through cell implantation in scaffolds depends mainly on the architecture of the scaffold and on the properties of the biomaterial used for their fabrication. The scaffold architecture is characterized by the pore shape and size, size distribution, pore interconnectivity and throat size. Among the polymers selected for tissue engineering, polyurethanes (PUR) represent a very important group. By varying the molecular weight of polyol and the composition of the hard segments, properties of PUR can be tuned for use in tissue engineering, either for reconstruction of soft tissue or for cartilage and bone regeneration.

The objective of this study was to characterize polyurethane porous scaffolds fabricated by the salt-leaching/polymer coagulation method. The effect of solution concentration and salt particles size on porosity and pore size distribution was evaluated by mercury-intrusion porosimetry and quantitative analysis of the scanning electron microscopy images. Mechanical properties of the scaffolds were measured in compression tests and by dynamic mechanical analysis (DMA). Growth of Human Bone Derived Cells (HBDCs) cultured in direct contact with the porous polyurethane scaffolds was investigated. In vitro studies in simulated body fluid were carried out to determine kinetics of the biodegradation process of polyurethane foams and the bioactivity of PUR-based scaffolds. Structure of the samples after soaking in SBF was characterized by scanning electron microscopy (SEM), EDS analysis and attenuated total reflectance–Fourier transform infrared spectrometry (FTIR–ATR).

Acknowledgements

This work was supported by the Ministry of Science and Higher Education, grant R13 01901.

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A degradable and highly porous polyurethane scaffolds for bone tissue engineering

Presentation: Oral at E-MRS Fall Meeting 2009, Symposium F, by Monika Bil See On-line Journal of E-MRS Fall Meeting 2009 Submitted: 2009-05-25 23:49 Revised: 2009-06-07 00:48

Presentation: Oral at E-MRS Fall Meeting 2009, Symposium F, by Monika Bil
See On-line Journal of E-MRS Fall Meeting 2009

Submitted: 2009-05-25 23:49 Revised: 2009-06-07 00:48