Poly(epsilon-caprolactone)- and poly(methyl methacrylate)-based scaffolds in human bone-derived cell cultures in vitro

Radosław M. Olkowski 1Katarzyna Filipczak Anna Chróścicka 1Piotr Woźniak 1Piotr Ulanski Janusz M. Rosiak Jacek Przybylski 1Małgorzata Lewandowska-Szumieł 1

1. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland

Abstract


Poly(methyl methacrylate) (PMMA) and poly(epsilon-caprolactone) (PCL) are synthetic polymers already used in medical applications. They are both taken into account as candidate scaffolds for cell transplantation in reconstructive surgery by means of tissue engineering. The aim of this work was to verify differences in morphology and vitality of Human Bone-Derived Cells (HBDC), cultured on PMMA and PCL. Polymers used for experiments were prepared in the form of 1mm high cylinders, 6.2mm in diameter, and placed at the bottom of a 96-well culture dish. Samples of PMMA were smooth, samples of PCL were either smooth or porous. Cells used for experiment were isolated from postsurgery bone tissue, which would be otherwise discarded. HBDC were cultured under standard conditions directly on the samples of PMMA and PCL and on the tissue culture polystyrene (TCPS) as a control. To assess HBDC vitality, XTT assay was performed. Morphological observations of CFDASE fluorophore stained cells in contact with biomaterials were carried out in reflected light and ultraviolet.
Vitality of HBDC cultured on the PMMA and PCL samples was found to be dependent on the mode of samples preparation and their porosity. Fluorescently stained HBDC cultured on both tested polymers were numerous and well spread. They differed in shape dependending on the support. Cells cultured on PCL were elongated and uniform; cells on PMMA had irregular shape.
Both biomaterials tested were found to be biocompatible in contact with human osteoblast-like cells. The influence of their surface and structure characteristics on cell function in vitro needs further investigations.

This work was supported by the State Committee for Scientific Research (grant 05/PBZ-KBN-082/2002/06).

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Presentation: oral at E-MRS Fall Meeting 2004, Symposium B, by Radosław M. Olkowski
See On-line Journal of E-MRS Fall Meeting 2004

Submitted: 2004-04-27 13:24
Revised:   2009-06-08 12:55
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