Poly(methyl methacrylate) (PMMA) and poly(epsilon-caprolactone) (PCL) are synthetic polymers already used in medical applications. They are both taken into account as candidate scaffolds for cell transplantation in reconstructive surgery by means of tissue engineering. The aim of this work was to verify differences in morphology and vitality of Human Bone-Derived Cells (HBDC), cultured on PMMA and PCL. Polymers used for experiments were prepared in the form of 1mm high cylinders, 6.2mm in diameter, and placed at the bottom of a 96-well culture dish. Samples of PMMA were smooth, samples of PCL were either smooth or porous. Cells used for experiment were isolated from postsurgery bone tissue, which would be otherwise discarded. HBDC were cultured under standard conditions directly on the samples of PMMA and PCL and on the tissue culture polystyrene (TCPS) as a control. To assess HBDC vitality, XTT assay was performed. Morphological observations of CFDASE fluorophore stained cells in contact with biomaterials were carried out in reflected light and ultraviolet.
Vitality of HBDC cultured on the PMMA and PCL samples was found to be dependent on the mode of samples preparation and their porosity. Fluorescently stained HBDC cultured on both tested polymers were numerous and well spread. They differed in shape dependending on the support. Cells cultured on PCL were elongated and uniform; cells on PMMA had irregular shape.
Both biomaterials tested were found to be biocompatible in contact with human osteoblast-like cells. The influence of their surface and structure characteristics on cell function in vitro needs further investigations.

This work was supported by the State Committee for Scientific Research (grant 05/PBZ-KBN-082/2002/06).

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/1756 must be provided.

1. Synthesis of highly biocompatible hydroxyapatite nanopowders
2. Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds
3.  A  degradable and highly porous polyurethane scaffolds for bone tissue engineering
4. A bit of physics in our understanding of the cell and tissue interaction with scaffolds
5. Culture of osteoblasts on nanostructured titanium – preliminary observation
6. Bone tissue engineered product based on human bone derived cells and polyurethane scaffold
7. Calcite-based material for tissue engineering ceramic scaffolds
8. Optimization of polyurethane structure as the potential materials for bone tissue engineering applications
9. Nanomechanical measurements of thin dextran layers
10. Cells and scaffolds for tissue engineering - what is needed for success?
11. Mechanical Properties of Human Plasma Derived Haemostatic Sponges Undergoing Radiation
12. Human Plasma Proteins as a New Haemostatic Material. Biocompatibility Testing.
13. Macroscopic and microscopic hydrogels synthesized by radiation technique and their selected biomedical applications
14. Modification of polymers by ultrasonic treatment in aqueous solution
15. Porous polymeric scaffolds for bone regeneration
16. Effect of diisocyanate chemical structure on biocompatibility of segmented polyurethanes
17. Mechanical characteristics of polymeric support as an important factor in osteoblast culture in vitro
18. Alginate hydrogel-candidate support for cell transplantation - preliminary observation in human chondrocyte culture
19. Omentum major as potential source of osteogenic cells for tissue engineering (preliminary report)
20. Differentiation of cells from adipose tissue into osteoblasts in the presence of biomaterials in vitro (preliminary report)
21. Alternative methods bring a new input in biocompatibility testing