Tissue engineering applies biological, medical and technical science for the sake of regeneration, maintenance and improvement of tissue functions. Studies concerning new materials for cells culture scaffolds are a quickly developing domain of biomedical engineering.

The goal of the present research consisted in optimization of calcite materials chemical composition and possibility of quantitative analysis of porous scaffolds by micro-CT.

Several materials based on CaCO₃ were elaborated. Microporous samples were formed by uniaxial pressing. For macroporous ones the method of mapping the porous structure of polyurethane sponges was applied. Apparent density, total porosity and compressive strength of tested samples were determined. The porous structures were observed in stereoscopic, SEM and analysed by micro-CT method.

The microporous calcite materials were characterised by total porosity ranging from 0,7 to 27%, apparent density between 1,8 and 2,5 g/cm³ and the compression strength ranging from 28 to 108MPa. These properties differed according to the chemical composition and sintering temperature.

The physical properties of macroporous calcite materials obtained by sponge method depended mainly on the technique of extrusion of the surplus slurry applied. The porosity of these materials ranged from 58 to 78%, apparent density from 0,55 to 1,07 g/cm³ and compressive strength from 0,5 to 2,0MPa.

The biological study also shown higher viability of the cells cultured on the samples with a lower content of technical additives.

The analysis of results demonstrated that relative density and total porosity obtained by microtomographic method match the values measured by geometrical method. Micro-CT analysis enables to calculate supplementary information, such as: volume and surface of the material, width and number of struts, and other parameters describing architecture of porous material.

This work was supported by Ministry of Science and Higher Education (grant nr R13 01901).

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