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Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds

Ida O. Dulińska-Molak ¹, Joanna Ryszkowska , Piotr Woźniak ², Małgorzata Lewandowska-Szumieł ², Krzysztof J. Kurzydlowski ¹

1. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland

Abstract

The tissue engineering scaffolds act as a substrate for cells, and their subsequent growth and proliferation. The basic requirements for the scaffold material are biocompatibility, degradability and osteoconductivity. This paper reports that such requirements are met by novel biodegradable scaffolds made of poly (e-caprolactone) urethane (PCL_PUR) porous matrix and calcium carbonate CaCO₃ (calcite and aragonite) developed for bone tissue engineering.

The polyurethanes were prepared by in situ polymerization. Porous structure of the scaffolds was obtained by the salt leaching/polymer coagulation method, using NaCl with the grain size 300-450 um as a porogen. The structures of composite materials were observed by scanning electron microscopy SEM and analysed by micro-CT. The mechanical properties of PUR/CaCO₃ composites were evaluated in static and dynamic compression test. The thermal properties of composites were investigated using Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimeter (DSC).

In vitro studies in simulated body fluid (SBF) were performed to study the influence of calcium carbonate (CaCO₃) on a bioactivity. Calcium phosphates formation in the form of the apatite layer on PUR/CaCO₃ composites was investigated byX-ray Photoemission Spectroscopy (XPS), Scanning Electron Microscope (SEM) equipped with Energy-Dispersive Spectrometer (EDS) and Fourier transformed infrared spectroscopy-attenuated total reflectance (FTIR-ATR). The structure composites materials was observed by scanning electron microscopy SEM and analysed by micro-CT.

Acknowledgements:The authors thank Dr Zbigniew Jaegermann from Institute of Glass and Ceramics for supplying us calcium carbonate. This scientific work was funded from the finances for education in the years 2006-2009 as research project no. R1301901

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Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds

Presentation: Poster at E-MRS Fall Meeting 2009, Symposium F, by Ida O. Dulińska-Molak See On-line Journal of E-MRS Fall Meeting 2009 Submitted: 2009-05-25 14:15 Revised: 2009-06-30 20:02

Presentation: Poster at E-MRS Fall Meeting 2009, Symposium F, by Ida O. Dulińska-Molak
See On-line Journal of E-MRS Fall Meeting 2009

Submitted: 2009-05-25 14:15 Revised: 2009-06-30 20:02