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Core-shell P(LLA-CL) based electrospun nanofibers for peripheral nerve tissue engineering

Ewa Kijeńska 1,2Dan Kai 1Molamma P. Prabhakaran 1Wojciech Swieszkowski 2Krzysztof J. Kurzydlowski 2Seeram Ramakrishna 1

1. Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore
2. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland


Unhealed peripheral nerve defects caused by inflammation or trauma are one of the major problems in today’s regenerative medicine. Existing methods of fracture treatment of longer gaps (more than 20 mm) requiring use of sensor autografts do not give a guarantee of full recovery and functionality of the nerve tissue. Schwann cells, which support neurons in peripheral nervous systems and are involved in conduction of nervous impulses along axons play a significant role in nerve tissue engineering. Laminin is an extracellular matrix (ECM) component synthesized after nerve injury and it plays a crucial role in cell migration and axonal growth. A chemical and biological composition of the engineered scaffolds along with its mechanical stiffness influence proliferation and spreading of the cells.  Electrospinning is a versatile and simple method, which allows to produce nanofibers with high surface area to volume ratio. Advance techniques such as coaxial electrospinning enables fabrication of nanofibrous scaffolds with bioactive agents encapsulated within the core of the fiber allowing its controlled delivery.

The aim of this study was to fabricate P(LLA-CL), blended P(LLA-CL)/Laminin and core-shell Laminin/P(LLA-CL) nanofibers and characterize their structure, morphology along with mechanical properties and biocompatibility towards Schwann cells. We obtained P(LLA-CL), blended P(LLA-CL)/Laminin and core-shell Laminin/P(LLA-CL) nanofibers with diameter of 302±101 nm, 350±112 nm and 316±110 nm respectively. The biological study confirmed that use of Laminin/P(LLA-CL) core-shell nanofibers increases proliferation and spreading of the Schwann cells compared to pure polymeric and blended nanofibers. Finally, it could be concluded that the obtained core-shell nanofibers show high potential to be a suitable substrate for peripheral nerve tissue engineering.


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Presentation: Poster at Nano-Biotechnologia PL, by Ewa Kijeńska
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-07-13 14:12
Revised:   2012-07-13 14:12