In this work authors present novel method of bone scaffolds fabrication. Matrixes are manufactured by chemical agglomeration of chitosan/β-TCP/collagen spheres and seem to be a promising bone tissue substitute. Fabrication process consists of the following steps: polymer based spheres extrusion, matrix formation by compression and spheres bonding with sodium tripolyphosphate (STPP) as cross-linking agent. Presented method allows to generate porous materials with controllable shape, pore size distribution and its interconnectivity. In this technique 3D scaffold porosity can be regulated by altering spheres diameter. Authors studied the influence of β-TCP, collagen and cross-linker concentrations on scaffold morphology, mechanical properties, enzymatic degradation rate (in presence of lysozyme), water absorption and osteoblast-like (MG-63) cells response. Influence of various chitosan molecular weights and length of STPP cross-linking on scaffold properties were also examined. Surface morphology and topography were evaluated by scanning electron microscope (SEM). Porosity and pores interconnectivity were observed via Micro-Computed Tomography (μCT) scanning. Mechanical tests showed that cross-linked chitosan performs compression characteristic (Young Modulus, maximum stress) similar to natural bone. Obtained matrices subjected to 10kN compression strength remain tough and not fractured. Cytotoxity evaluated by XTT assay shows that proposed composite materials don’t exhibit any toxic properties against human cells. Osteoblast-like cells adhesion and morphology were analyzed both by SEM and optical microscope.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/16118 must be provided.

1. Joining ultrafine grained aluminium by friction stir welding - processing, microstructure and mechanical properties
2. Electrospun nanocomposites from PHBV for bone tissue engineering
3. Biomimetic coatings for implants
4. Application of micro- and nanostructured biomaterials in regenerative medicine
5. Core-shell P(LLA-CL) based electrospun nanofibers for peripheral nerve tissue engineering
6. Polysaccharide nanoparticles for anticancer drug delivery 
7. Biomimetic medical coatings with improved hemocompatibility
8. Synthesis of highly biocompatible hydroxyapatite nanopowders
9. Solvothermal synthesis of nonstoichiometric hydroxyapatite nanoparticles
10. Solvothermal synthesis of nonstoichiometric hydroxyapatite nanoparticles
11. Particles for controlled drug delivery
12. Micro Sensor for cell force measurement
13. Composites PLLA/MWNTs produced by twin-screw microcompounder
14. Investigation of the DLC and novel DLC-polymer hybrid coatings using FIB and SEM techniques
15. A combination of microCT and FEM methods for development of a new scaffold for bone regeneration
16. Investigation of fatique crack growth rate of Al 5484 ultrafine grained alloy after ECAP process
17. Electrospun polymeric fibers for cartilage regeneration
18. Biodegradable and bioabsorbable polymeric - ceramic composites for medical applications
19. Preliminary examinations of mechanical properties of DLC and novel DLC-polymer hybrid coatings for resurfacing hip prostheses
20. Optimization of the polymeric scaffolds for bone tissue engineering fabricated by rapid prototyping method
21. Hydrogel coatings and a local drug delivery
22. Electrically enhanced and controlled drug delivery through buccal mucosa
23. Repair and regeneration of osteochondral defects in the articular joints