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Electrospun nanocomposites from PHBV for bone tissue engineering

Katarzyna Zalewska-Wierzbicka ,  Joanna Idaszek ,  Ewa Kijeńska ,  Wojciech Swieszkowski ,  Krzysztof J. Kurzydlowski 

Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland


Filling the defects which are caused by fractures, injuries or surgical interventions after tumor resections are of the most importance in today’s regenerative medicine. One of the promising technologies for scaffold fabrication is electrospinning. This method allows for micro and nanofibers preparation from polymer solutions with the use of electrostatic forces1. Electrospun nanostructure scaffolds composed of biodegradable polymer systems could not only mimic a structure of native Extracellular Matrix (ECM), but also work as a carrier for control release of drugs into injured site.

The aim of this study was development and characterization of nanofibrous mats from novel biodegradable polymer, extracted from biomass, poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) blended with poly-3-hydroxybutyrate (PHB) and Bovine Serum Albumin (BSA). Polymeric composites based on polyhydroxyalkanoates have drawn considerable attention in recent years as a scaffolding material in tissue engineering and regenerative medicine due to their degradation ratio and non-toxic product hydrolysis (R)-3-hydrobutyric acid which serves as a normal constituent of human blood. BSA was used as a model protein. Cytocompatibility of the prepared mats was evaluated. The topography and morphology of the obtained nanofibers mats was analaysed using a Scanning Electron Microscopy (SEM). The wettability of the mats was measured by water contact angle. Mechanical properties were investigated using a mechanical tester. Release study of BSA were evaluated using spectrophotometer UV-Vis.

The process parameters of electrospinning were optimized in order to produce uniform fibers from pure polymer and  polymeric composites. The obtained fiber diameter decreased with addition of PHB. The fibers diameters were up to 500 nm. Our results present the effects of PHB and BSA addition to PHBV polymer blends and its influence to Human Mesenchymal Stem Cells (hMSC) response.

This work was financed by National Science Centre on the basis of a decision number DEC-2011/01/M/ST8/07742.

1.Y.Ito et al., Journal of Bioscience and Bioengineering 2005,100,43


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Presentation: Poster at Nano PL 2014, Symposium B, by Katarzyna Zalewska-Wierzbicka
See On-line Journal of Nano PL 2014

Submitted: 2014-06-26 01:01
Revised:   2014-09-26 14:10