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CLA derivatives as functional food compounds

Wiesława Walisiewicz-Niedbalska 1Andrzej W. Lipkowski 1,2Joanna Wietrzyk 3Bożena Patkowska-Sokoła 4Krzysztof M. Różycki 1

1. Industrial Chemistry Research Institute (ICRI), Rydygiera 8, Warszawa 01-793, Poland
2. Polish Academy of Sciences, GBAF, Medical Research Center (CMDiK PAN), Pawinskiego 5, Warszawa 02-106, Poland
3. Polish Academy of Sciences, Institute of Immunology and Experimental Therapy (IITD), Rudolfa Weigla 12, Wrocław 53-114, Poland
4. Wrocław University of Environmental and Life Sciences, Chełmońskiego 38d, Wrocław 51-630, Poland


The work presents the results of the study on a synthesis of biologically active isomers of linoleic acid 9c,11t and 10t,12c C18:2 (CLA conjugated linoleic acid) obtained from poppy seed oil, as well as the results of the study on the influence of preparation obtained on the size of breast cancer tumour nodule.

The aim of the work was to establish the method of biologically active isomers of linoleic acid (9c,11t and 10t,12cC18:2) obtaining from poppy seed oil rich in linoleic acid (9c,12cC18:2) and to determine the influence of that isomers on size changes of breast cancer tumour nodule.

The raw research material was poppy seed oil containing about 72% of linoleic acid (9c,12cC18:2) bounded in triacylglycerols. The isomers of linoleic acid were obtained by the method of positional and geometric isomerisation of linoleic acid. Poppy seed oil isomerisation was conducted in the presence of alkaline catalyst in propylene glycol or glycerin environment. Qualitative identification and quantitative analysis were conducted using the method of gas chromatography with standards of 9c,11tC18:2 and 10t,12cC18:2 isomers of Sigma company. Saponification of triacylglycerols of poppy seed oil to free fatty acids took place concurrently to the reaction of positional and geometrical isomerisation.

The application study of the product obtained was conducted on mice with orthotropically injected cells of breast cancer 16/c. Volume of nodule in mm3 was studied and compared to the control sample.

As a result of the study conducted, the method of isomerisation of linoleic acid, the main component of poppy seed oil, was established obtaining the product containing mainly 9c,11tC18:2 (about 35%) and 10t,12cC18:2 (about 36%) isomers. Basing on the application study of the product obtained its influence on an inhibition of development of breast cancer tumour nodule of about 30% on average may be concluded.

The results obtained point the possibility of linoleic acid isomers obtaining from poppy seed oil, and their biological activity towards breast cancer tumour nodules. Since the mixture of CLA isomers was used for the study, it seems to be reasonable to work out a successful method of isomers separation and to obtain the two fractions containing separately both isomers 9c,11t and 10t,12c. Similar study should be conducted for such obtained products in order to determine biological activity of both isomers towards wider group of tumour nodules. As a target, the preparations obtained containing isomers of linoleic acid would be used as functional food components.


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Related papers

Presentation: Poster at VII Multidyscyplinarna Konferencja Nauki o Leku, by Wiesława Walisiewicz-Niedbalska
See On-line Journal of VII Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2010-03-22 09:55
Revised:   2010-04-08 09:27