ANTICANCER PROPERTIES OF HAIR PROTEINS DIGESTIVE PEPTIDES

Joanna Matalinska¹, Andrzej W. Lipkowski¹, Sergiusz Markowicz², Katarzyna Kurzepa³, Marta Bochynska¹, Marzena Biernacka², Anna Samluk², Dorota Dudek², Henryk Skurzak²

¹ Mossakowski Medical Research Centre, Warsaw, Poland

² Maria Sklodowska-Curie Oncological Research Centre, Warsaw, Poland

³ Industrial Chemistry Research Institute, Warsaw, Poland

Mechanical and thermal protection of the body is believed to be the major function of hair and fur that cover mammalian skin. Proteins that construct hair are characterized by extremely high resistance for degradation. However, it should be taken into account that even such environmentally resistant material may be also slowly broken down by mechanical wiping or biodegradation due to skin endogenous enzymes or hosting of numerous microbes. Similar processes may take place not only in skin, but also in stomach due to well known phenomena of fur skein swallowing by animals. We hypothesized, that biodegradation products of hair or fur may possess bioprotective properties which are supplementary to the physical hair protective features. The evolutionary process resulted in a reduced hair-covered space on human body, but it may be assumed that bioprotective effects of hair degradation products persist. We applied the process of partial enzymatic digestion of hair and fur with pepsin. Enzymatic digestive water soluble lysate consists of mixture of peptides, including fragments of keratins and keratin associated proteins. Human skin is exposed for various environmental cancerogenic factors. Therefore, we hypothesized that bioprotective mechanisms of hair lysate, if any, could affect proliferation of melanoma cells. Indeed, we found that mixtures of soluble peptides originated from human hair inhibited proliferation of human melanoma cells in vitro. The inhibitory effect of such peptide mixtures on B lymphoma cells and urinary bladder cancer cells was also observed. Normal human cells populations varied in their susceptibility to lysate effects. Hair-originated peptide mixtures inhibited proliferation of normal human fibroblasts, but did not affect proliferationof human mesenchymal cells. Identification of active components of hair and mechanisms of their action may delineate a new avenue for anticancer drug development.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28127 must be provided.

1. Sex differences in the opioid component of swim stress-induced analgesia in mouse lines selected for high or low sensitivity to stress
2. Efficacy of Viburnum opulus fruit juice and its components against herpesvirus
3. New analgesics with adjuvant anticancer properties.
4. Keratin Associeted Proteins as a New Type of Bandage for Wound Healing
5. Development of new multitarget GPCRs ligands (opioid agonist- tachykinin antagonist).
6. Effects D-tryptophan substitutions on the activity and toxicity of antimicrobial dendrimers
7. Novel amphiphilic dendrimers as antibacterial and antitumoral agents.
8. CLA derivatives as functional food compounds
9. Keratin associated proteins (KAPs) as a new biomaterials for applications in medicine and cosmetology
10. Natural compositions with antioxidative properties
11. Application of fermentation in protein hydrolysates utilisation 
12. Comparable antinociceptive effect of biphalin in mice selected for high and low swim stress-induced analgesia after blood-brain barrier disruption caused by hyperosmolar mannitol administration
13. Application of preparative HPLC for protected peptide purification
14. New tachykinin-opioid chimeric analogues as potential new analgesics
15. Spinal cord peptide epitopes ameliorate immunological reaction in  experimental allergic encephalomyelitis /eae/
16. Dendrimeric peptides with  affinity to opioid receptors - complexation properties
17. Development of dendrimeric somatostatin analogues
18. H-Dmt-D-Lys-Phe-Phe-OH, a tetrapeptide metabolite of the opioid-neurotensin hybrid peptide PK20, expresses high antinociception
19. Harnessing stem cells and dendritic cells for novel therapies
20. THERMOSENSITIVE TRI-BLOCK-CO-POLY- MERS POLY- (HPMAm-MONO/DI LACTATE)- PEG-POLY(HPMAm-MONO/DI LACTATE) FOR THE CONTROLLED RELEASE OF OPIOID PEPTIDES
21. HYDROLISATE OF PIG SPINAL CORD AS A NUTRACEUTIC IN MULTIPLE SCLEROSIS.
22. KERATINE PREPARATIONS FOR WOUND HEALING
23. DEVELOPMENT OF NEW TACHYKININ-OPIOID CHIMERIC ANALOGUES AS POTENTIAL NEW ANALGESIC
24. OPIOID PEPTIDE ANALOGUE AA2016 IN MOUSE MODEL OF INDIVIDUAL PAIN SENSITIVITY
25. A SIMPLE PREPARATIVE HPLC ISOLATION OF CONJUGATED LINOLEIC ACIDS (CLAs).
26. SEARCH FOR NEW ANTIMICROBIAL PEPTIDES FROM HYDROLIZATES OF SEED PROTEINS.