Recent studies of gut-blood barrier have showed that short peptides of food proteins are transported to the blood easier than aminoacids. Special system presenting antigens in gut is formed. This process presenting food peptides and suppressing of immunological respons to them is called oral tolerance. Recently, it has been proposed to apply it to the treatment of autoimmune diseases as multiple sclerosis, rheumatoid athritis, uveitis and diabetes type 2. The aim of our study was to use hydrolisate of pig spinal cord proteins which is the mixture of neuropeptides obtained as a result of hydrolisis of an undenatured homogenate of proteins as an antigen for feeding experimental animals/rats/.After induction of oral tolerance, animals were immunised by injection of guine pig spinal cord homogenate with complete Freund s adjuvant to evoke experimental allergic encephalomyelitis /EAE/, which is an animal model of multiple sclerosis. Clinical course was observed, histopathological study, ultramicroscopic study and metaloproteases determination in the brain was done.Results. Clinical course of EAE post hydrolisate treatment has been milder than control one. MBP and TNF alfa in the brain were decreased. Metaloproteases increased in EAE, after hydrolisate treatment were diminished by 30 %. Some changes in blood brain barrier/BBB/ as opened tight junction and other changes in early phase of EAE as kariosceletal damage, compartmentalisation of the endoplasmic reticulum, large cisterns of the Golgi apparatus, increased activity of microglial cells with numbers of phagolisosomes, desorganisation of sheets of myelin, neoangiogenesis of parenchyma of the cerebral cortex , have been dimished. Above results indicate on possible clinical use of oral tolerance induced by pig spinal cord hydrolisate as a supportive treatment of multiple sclerosis.

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