Conjugated linoleic acid (CLA), a natural polyunsaturated fatty acid, is one of the major components of nutraceutics, mainly taking into account the terapeutical applications; many studies have shown that CLA may play an important role in helping fight obesity, cancer, diabetes, as well as hardening of the arteries.

In natural occurring products a very low concentration (≤ 7%) of this fatty acid is noticed, therefore it is necessary to isolate it while nutraceuticals production. However, every method of the isolation of conjugatd linoleic acid is rather arduous and expensive (e.g by super-critical extraction connected with crystallization using urea we are able to achieve only 11% of CLA, which is the maximum value). Thus it seems to be crucial to find the proper method which will be efficient, especially during the large-scale isolation process.

In general, preparative HPLC, due to its costs, it is not considered as an economical method which might be used in nutraceutics isolation. However, we have developed a simple HPLC method with the implementation of methanol, in which we are able to obtain as well as separate "blocks" of various fatty acids, including conjugated unsaturated fatty acids.

This method may be used for the preparative isolation of conjugated unsaturated FA (diens or triens) derived from either natural sources (milk, oils) or chemical izomerization products.

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