Nutraceutics became important components of successful therapies. Recently particular attention has been focused on linoleic acid isomers (CLA) containing conjugated double bonds.

Conjugated linoleic acids (CLA) represent a family of octadecadienoic acids which constitute a mixture of geometric and position isomers of linoleic acid in which the double bond occurs in the positions ranging from 6,8 to 9,11 and to 10,12. The 9c,11t C_18:2 acid is the predominant isomer.

The content of the 9c,11t C_18:2 isomer in the cow milk fat, goat milk fat and sheep milk fat were found to oscillate within the ranges 0.65-1.8%, 0.3-1.2% and 1.2-2.3%, respectively. The sheep milk fat shows the highest content of the isomer.

Sheep milk fat containing 2% of the 9c,11t C_18:2 conjugated linoleic acid (CLA) isomer was selected to study the antiproliferative activity of this isomer. Sheep milk fatty acids containing 2.0% of the 9c,11t C_18:2 isomer, and the acids enriched in this isomer up to 12.1%, (N-CLA), were used in the studies.

The product (N-CLA) contained 15.0% of the 9c,11t C_18:2 isomer and about 8.0% of the 11t C_18:1. The sheep milk fatty acids composition and the product enriched in CLA (N-CLA), and also a commercial CLA preparation, were studied in antiproliferative activity against few lines of human cancer.

The effect of CLA-enriched preparation of sheep milk fatty acids, N-CLA, revealed higher antiproliferative activity than standard pure CLA and sheep milk fatty acids.

The present study was carried out in the framework of the Research Projects 3 T09 B 059 26 financed by the Ministry of Education and Science.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/6943 must be provided.

1. Synthesis of new 5H-indolo[2,3-b]quinoline derivatives with a high selective cytotoxic activity
2. Neuroprotective effects of biphalin in a mouse model of mild traumatic brain injury (mTBI)
3. Administration of isothiocyanate-derived mercapturic acids inhibits solid tumor growth of 4T1 murine mammary carcinoma cells in BALB/c mice
4. Preliminary studies on application of library of artificial receptors for differentiation of metabolites in urine of healthy and cancer mice
5. Starch derivatives as vehicles for drug delivery in anticancer therapy
6. Mercapturic acids as a naturally occurring isothiocyanates prodrug form with site-directed delivery system built-in based on an oxidative environment of the tumor
7. Synthesis and biological activity of novel N,N-cyclic-2,4-dihydroxythiobenzamide derivatives
8. Anticancer activity of vitamin D, genistein and indoloquinoline analogs
9. Synthesis and anticancer activity of acetylenic derivatives of betulin
10. Interaction of endotoxin and polymyxin B in B16 mouse melanoma model of metatstasis
11. Anticancer activity of the conjugates:  modified fibrinogen – methotrexate.
12. Synthesis and cytotoxic activity of new Pt (II) complexes with nitropyrazoles in aerobic and hypoxic conditions
13. Vitamin D analogs enhance the activity of imatinib mesylate in human non-small cell lung cancer model
14. Colon cancer treatment with the use of vitamin D analogs and irinotecan 
15. Novel bifunctional synthetic isothiocyanates with high antiproliferative activity
16. Naturally occurring hydroxyalkyl isothiocyanates and isothiocyanatoalkyl benzoates - structure-activity and structure-mechanism of action relationship studies.
17. Synthesis and biological evaluation of new amino acid and dipeptide derivatives of neocryptolepine as anticancer agents. 
18. Antitumor activity of carrier-methotrexate conjugates.
19. CLA derivatives as functional food compounds
20. New acetylenic derivatives of betulin with anticancer activity
21. Catalytic synthesis and antiproliferative activity in vitro of polisubstituted isoxazolopyridine derivatives.
22. Assessment of analgesic potency of biphalin in a mouse model of cancer pain  
23. Steroid profile of Asplenium cuneifolium
24. The influence of (24R)-1,24- dihydroxyvitamin D₃ on the anticancer activity of 5-fluorouracil in human colon cancer model
25. Comparison of antiproliferative activity of the natural isothiocyanates and their mercapturic derivatives.
26. Could the calorically restricted ketogenic diet be an     effective alternative or supportive therapy for breast cancer?
27. Analogs of genistein and its possible anti-metastatic properties
28. The influence of novel analogs of vitamin D₃ on the antiproliferative activity of cisplatin or doxorubicin on human promyelocytic leukaemia cell line HL-60 
29. New amino acid and peptide derivatives of 5H-indolo[2,3-b]quinoline and their biological activity.
30. The cytotoxic activity of glycosides of indolo[2,3-b]quinoline derivatives.
31. Disubstituted indolo[2,3-b]quinoline derivatives - the cytotoxic activity in vitro against various human tumor cell lines.
32. Mono substituted 5H- and 6H-indolo[2,3-b]quinoline derivatives and their ability to overcome the barrier of drug resistance.
33. Synthesis and antiproliferative activity in vitro of (4-substituted-2-butynylthio)quinolines
34. New calcipotriol analogs, their toxicity and antitumor activity in vivo in comparison to the affinity with VDR and DBP
35. Synthesis and cytostatic activity of new 1-substituted 6H-pyrido[4,3-b]carbazole derivatives
36. The new analogs of genistein decrease mitochondrial membrane potential and activate caspase-3
37. New amino acid derivatives of 6H-indolo[2,3-b]quinolines
38. Influence of Transformation of the Amino Group in Anthracyclines into -N=CH-N< or -N=CH-O- Group on Their Biological Properties
39. PRIMARY CYTOTOXICITY EVALUATION OF NEW HETEROCYCLIC SYSTEMS WITH PYRIDODIAZEPINE.
40. SYNTHESIS AND ANTIPROLIFERATIVE ACTIVITY IN VITRO OF NEW 2-AMINOBENZIMIDAZOLE DERIVATIVES. PART 3. REACTIONS OF 2-ARYLIDENE- AMINOBENZIMIDAZOLE WITH SELECTED 1,3-DIKETONES.
41. A SIMPLE PREPARATIVE HPLC ISOLATION OF CONJUGATED LINOLEIC ACIDS (CLAs).
42. THE ANTIMETASTATIC EFFECT OF BACTERIOPHAGE T4* ADMINISTRATED IN COMBINED TREATMENT WITH SELECTED CYTOSTATICS IN MICE WITH B16 MELANOMA
43. SYNTHESIS AND ANTIPROLIFERATIVE ACTIVITY OF DIACETYLENIC THIOQUINOLINES
44. SYNTHESIS AND CYTOTOXIC ACTIVITY OF 10-DEACETYL-10-PROPARGILPACLITAXEL
45. THE CYTOTOXIC GLYCOSIDES OF INDOLO[2,3-B]QUINOLINE DERIVATIVES. IN VITRO AND IN VIVO STUDIES
46. MONO SUBSTITUTED 5H-INDOLO[2,3-B]- QUINOLINE DERIVATIVES AND THEIR ABILITY TO OVERCOME THE BARRIER OF DRUG RESISTANCE.
47. DISUBSTITUTED INDOLO[2,3-b]QUINOLINE DERIVATIVES. THE CYTOTOXIC ACTIVITY IN VITRO AGAINST VARIOUS HUMAN TUMOR CELL LINE.
48. ANTITUMOR EFFECT OF CISPLATIN OR FLUOROURACIL IN COMBINED TREATMENT WITH (24R)-1,24-DIHYDROXYVITA- MIN D₃
49. SYNTHESIS AND CYTOTOXIC ACTIVITY OF NEW 1-SUBSTITUTED PYRIDO[4,3-b]CARBA- ZOLE DERIVATIVES
50. FIRST ROUND STUDIES ON LEWIS LUNG CARCINOMA ACTIVITY OF THE NEW LEAD STRUCTURES IN THE ISOXAZOLE HETEROCYCLIC SYSTEM
51. CONJUGATES OF METHOTREXATE AND DEXTRANS IN MOUSE LEUKEMIA MODEL WITH MULTIPLE DOSE SCHEDULE
52. GENISTEIN, ITS NEW ANALOGS AND COMPLEXES - THE ANTIPROLIFERATIVE EFFECTS ON HL-60 LEUKEMIA CELL LINE
53. COMBINATION OF: IMATINIB, PRI-2191 AND CYTOSTATICS - THE ANTIPROLIFERATIVE EFFECTS ON HL-60 LEUKEMIA CELL LINE
54. ANTIPROLIFERATIVE ACTIVITY IN VITRO OF DIASTEREOMERIC ANALOGUES OF VITAMIN D3 AGAINST NORMAL AND CANCER CELL LINES