The cytotoxic activity in vitro of glycosides of  indolo[2,3-b]guinoline ( 5H and 6H series) were tested against cervix carcinoma cell line (KB).

The cells were placed in 96-well flat-bottom plates at a density of 1x10⁴ cells per well in 100 ml of cultured medium 24 hours before addition of the tested compounds. The cells were exposed to the test compounds at concentrations 0.1, 1, 10, and 100 mg/ml for 72 h. The in vitro cytotoxic effect of all agents was examined using the SRB assay.

We found that all tested indoloquinolines showed cytotoxic activity against KB cells and the most potent were two of them: IBR-15 (ID50: 0,29±0,12 mg/ml) and IBR-17 (ID50: 0,33±0,08 mg/ml). The  5H serie was more active against cervix carcinoma cell line than  6H one.

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