Ultradźwiękowa technika pokrywania polimerowych rusztowań dla zastosowań biomedycznych

Aleksandra E. Kędzierska 1Barbara D. Ostrowska 2Dariusz Smolen 1Elżbieta Pietrzykowska 1Wojciech Swieszkowski 2Aharon Gedanken 3Witold Łojkowski 1

1. Polish Academy of Sciences, Institute of High Pressure Physics (UNIPRESS), Sokolowska 29/37, Warszawa 01-142, Poland
2. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
3. Bar-Ilan University, Ramat-Gan 52900, Israel

Abstract

Rusztowania przeznaczone do regeneracji kości umożliwiają wzrost kości w przypadku, gdy ubytek jest zbyt duży, by mógł być zaleczony w sposób naturalny. Takie sytuacje są często spotykane w przypadku złamań, po resekcji nowotworu czy też chirurgii kości twarzoczaszki lub szczęki. Istniejące obecnie na rynku materiały nie spełniają wymagań stawianych przez lekarzy. Proces regeneracji zajmuje zbyt dużo czasu, przestrzeń ubytku wypełniana jest nie dość dokładnie, a ich mechaniczne właściwości są zbyt słabe. W praktyce często pojawiają się też procesy zapalne w miejscu ubytku, co utrudnia lub uniemożliwia proces gojenia.

Ludzka kość jest zbudowana w części przez kryształy hydroksyapatytu. Jednak hydroksyapatyt syntetyczny, dostępny na rynku, jest materiałem uznanym za nieresorbowalny przez organizm ludzki. W Laboratorium Nanostruktur dla fotoniki i nanomedycyny IWC PAN opracowano proces mikrofalowej syntezy, który umożliwia otrzymanie w pełni resorbowalnego nano-hydroksyapatytu „GoHAp”.

Testy rozpuszczalności proszku GoHAp, przeprowadzone zgodnie z Europejskimi Standardami ISO 10993-6: 2007 wykazały, że ma on dużo wyższą rozpuszczalność w porównaniu do komercjalnie dostępnego proszku hydroksyapatytu (NanoXIM). Struktura materiału analizowana była za pomocą transmisyjnej mikroskopii elektronowej (TEM) (Rys.1), a także z pomocą metod dyfrakcji rentgenowskiej. Otrzymano kryształy proszku o morfologii płytkowej, wielkości krystalitów od 5 do 30 nm, oraz powierzchni właściwej do 240 m2/g.

W ramach przeprowadzonych badań polimerowe rusztowanie pokryto nanoproszkiem hydroksyapatytu GoHAp z wykorzystaniem techniki ultradźwiękowej. Warstwa taka umożliwi narastanie komórek kostnych na jego powierzchni z powodu wysokiej biozgodności proszku.

Uzyskaną warstwę analizowano za pomocą skaningowej mikroskopii elektronowej (SEM) (Rys.2.), mikroskopii sił atomowych (AFM) oraz mikroanalizy rentgenowskiej (EDX). Powyższymi metodami określono teksturę, skład oraz wytrzymałość połączenia ceramika/polimer. Wyznaczono także wartość kąta zwilżania powierzchni dla wody. Na podstawie pomiaru metodą atomowej spektrometrii emisyjnej (ICP) oznaczono całkowitą zawartość naniesionego nanoproszku.

Rys. 1. Zdjęcie TEM nanoproszku hydroksyapatytu (GoHAp), otrzymanego w Instytucie Wysokich Ciśnień PAN.


Rys. 2. Zdjęcie SEM polimerowego rusztowania pokrytego nanoproszkiem hydroksyapatytu.

Auxiliary resources (full texts, presentations, posters, etc.)
  1. POSTER: Ultradźwiękowa technika pokrywania polimerowych rusztowań dla zastosowań biomedycznych, PDF document, version 1.4, 2.8MB
 

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/28362 must be provided.

 

Related papers
  1. Optical oxygen sensor for safe operation in hazardous areas
  2.   Optical oxygen sensor for safe operation  in hazardous areas
  3. Quo Vadis nanotechnology- for various stakeholders
  4. Opening of the Thursday Sessions of NanoPl 2014
  5. Microwave reactors for nanoparticle's synthesis
  6. Opening of Nano-Bio-Med session
  7. Bioactive GoHAP nanoparticles and their applications in medical implants technology
  8. Wykorzystanie metody NTA (Nanoparticle Tracking Analysis) w ocenie wielkości krążących mikrocząstek osocza i innych płynów fizjologicznych: pułapki i triki
  9. Advanced characterisation of nanomaterials according to ISO 17025 Norm

  10. Microwave solvothermal synthesis of resorbable nano-HAp with controlled grain size.
  11. Electrospun nanocomposites from PHBV for bone tissue engineering
  12. Microwave Solvothermal Synthesis of Nano Zinc Oxide Nanoparticles
  13. Microwave Solvothermal Technology (MSS) for nanoparticle synthesis
  14. Zaawansowana Charakteryzacja Nanocząstek
  15. [CEPT] Solvothermal synthesis nano zinc oxide in  microwave reactor MSS2
  16. [CEPT] Solvothermal synthesis and characterization of nanomaterials in the Laboratory of Nanostructures for Photonics and Nanomedicine, Center of Bio-Nanomaterials
  17. Scanning Electron Microscopy Investigations and Analysis of Nanostructures
  18. Polish Nanotechnology Platform - a discussion group at Linked-In
  19. Novel optical oxygen sensor for life and health protection made of nano-zirconia
  20. Nanometrology as a tool for nanotechnology problems solving
  21. Creating a Polish Nano-Bio-Technology and  Nano-Medicine Platform?
  22. MSS synthesis of highly biocompatible nano-HAP and coating of 3D poymer scaffolds
  23. Nanotechnologie w gospodarce województwa podlaskiego
  24. Characterization of nanomaterials according to EU recommendations, on the example of ZnO nanoparticles
  25. [CEPT] Nanohydroxyapatite-polylactide composites  for regenerative implants
  26. [CEPT] MSS synthesis of highly biocompatible nano-HAP with properties depending on the synthesis time.
  27. [CEPT] Studies of nanoparticles size distribution
  28. [CEPT] Studies of solubility of nanoparticles and stability of their suspension
  29. Iinvestigation of nanoparticles by DSC-TG methods accompanied by chemical analysis of emitted gas
  30. Coating anti-bacterial nanoparticles on flat and curved surfaces, and fighting resistant bacteria by employing the sonochemical method
  31. Presentation of the  Laboratory of Nanostructures for Photonics and Nanomedicine, Center of Bio-Nanomaterials, CePT
  32. ZnO nanoparticles effect on integrity of human endothelial cells
  33. Importance and trends in nanomedicine according  European Nanomedicine Platform survey
  34. Application of the Zeiss Ultra Plus scanning microscopy in bio-nanotechnology 
  35. Application of micro- and nanostructured biomaterials in regenerative medicine
  36. Welcome address
  37. Core-shell P(LLA-CL) based electrospun nanofibers for peripheral nerve tissue engineering
  38. Otwarcie NanoInfoDay, Powitanie
  39. Characteristic of hydroxyapatite dense nanoceramic produced by The High Pressure Consolidation  
  40. Wpływ parametrów materiałowo-technicznych na właściwości optycznego czujnika tlenu na bazie nano-ZrO2
  41. Synteza nano-ZnO przy pomocy reaktora Mettler Toledo w Laboratorium Nanostruktur dla Fotoniki i Nanomedycyny CePT.
  42. Infrastruktura i specjalizacje nanotechnologii w Polsce na podstawie wyników ankiety NANOFORCE
  43. Solwotermalna synteza domieszkowanego nanotlenku cynku dla projektu NanoFATE. Otrzymywanie wodnych nanozawiesin domieszkowanego tlenku cynku.
  44. Nanotechnologia bezpieczna dla ludzi i przyjazna środowisku
  45. Badania rozkładu wielkości cząstek i potencjału zeta w Laboratorium Nanostruktur dla Fotoniki i Nanomedycyny CePT
  46. Badanie stabilności i rozpuszczalności zawiesin z nano ZnO i nano ZnO:Co otrzymanych w  Laboratorium Nanostrutur dla Fotoniki i Nanomedycyny CePT
  47. Badania nanoproszków przy pomocy technik DSC-TG/ QMS-FTIR w Laboratorium Nanostruktur dla Fotoniki i Nanomedycyny CePT
  48. Synthesis of highly biocompatible hydroxyapatite nanopowders
  49. Nanosafety - challenge of nowadays
  50. Influence of heat treatment on luminescence of ZrO+ Eu nanopowders
  51. Solvothermal synthesis of doped nano zinc oxide for the project NanoFATE.
  52. Podsumowanie
  53. Wet chemical methods of manufacturing alumina nanopowders
  54. Solwotermalna synteza domieszkowanego nano tlenku cynku dla projektu NanoFATE.
  55. Hydrothermal Synthesis of Zinc Oxide Nanopowders
  56. Optyczny sensor tlenu na bazie nanokrystalicznego ZrO2
  57. Solvothermal synthesis of nonstoichiometric hydroxyapatite nanoparticles
  58. Solvothermal synthesis of nonstoichiometric hydroxyapatite nanoparticles
  59. Procedury charakteryzacji nanoproszków
  60. Oferty nanoproszków - Laboratorium Nanostruktur, Instytut Wysokich Ciśnien PAN
  61. Nanofotonika dla małych i średnich przedsiębiorstw - mapy dorogowe i analizy SWOT  najważniejszych technologii.
  62. Otwarcie konferencji
  63. Polska  Platforma Nanotechnologii i zarys strategicznego programu "Nanotechnologia i wzrost inwestycji w B&R w Polsce"
  64. Nowe mikrofalowe reaktory do syntez nanocząstek - wybrane zagadnienia badawcze i przykłady zastosowań
  65. Micro Sensor for cell force measurement
  66. ZrO2:Tb nanopowders obtained by coprecipitation method
  67. Investigation of the DLC and novel DLC-polymer hybrid coatings using FIB and SEM techniques
  68. A combination of microCT and FEM methods for development of a new scaffold for bone regeneration
  69. ZnFe2O4/ZnO core-shell particles obtained by coprecipitation route
  70. Hydrothermal Synthesis of ZnAl2O4 Spinel
  71. Culture of osteoblasts on nanostructured titanium – preliminary observation
  72. Electrospun polymeric fibers for cartilage regeneration
  73. Biodegradable and bioabsorbable polymeric - ceramic composites for medical applications
  74. Preliminary examinations of mechanical properties of DLC and novel DLC-polymer hybrid coatings for resurfacing hip prostheses
  75. Optimization of the polymeric scaffolds for bone tissue engineering fabricated by rapid prototyping method
  76. Localization of rare-earth dopants in the lattice of nanocrystalline ZrO2 - EXAFS study
  77. Advanced nanoporous yttria-stabilized zirconia ceramics for luminescent oxygen sensors
  78. Novel method for bone tissue scaffold fabrication. Influence of composition chitosan/beta-TCP matrixes on mechanical and biological properties.
  79. Excitation transfer in zirconia nanocrystals and ceramics
  80. Eu Luminescence in zirconia nanocrystals
  81. ZnO ceramic sintering and luminescence properties
  82. Coating Flat and Curved Surfaces by Nanoparticles Using Ultrasound and Microwave Radiations
  83. Oferta zaawansowanych materiałów w perspektywie 5 - 20 lat
  84. Strategia rozwoju badań nanoproszków
  85. Error bars in powder diffraction
  86. Synthesis of doped ZnO nanopowders in microwave hydrothermal reactors
  87. Characterization of nanopowders
  88. Synthesis of Al doped ZnO nanopowders and their enhanced luminescence
  89. Synthesis of nanopowders in supercritical water in a continuous flow reactor
  90. A summary of results obtained in Working Group 002 "Synthesis and Processing of Nanopowders"
  91. Producing metal oxide nanoparticles by the sonochemical, microwave and RAPET techniques.
  92. Summary of activities of Working Group D30/003 "Synthesis and Processing of Nanopowders"
  93. Doped zirconia nanopowders made in microwave – pressure reactor
  94. Combining microwave and pressure techniques for hydrothermal synthesis of ZnO and ZrO2 nanopowders doped with a range of metal ions
  95. Luminescence properties of zinc oxide nanopowders doped with Al ions obtained by the hydrothermal and vapour condensation methods.
  96. Mechanical property of nano-TiO2 dispersed Al65Cu20Ti15 amorphous/nanocrystalline matrix bulk composite prepared by mechanical alloying and high pressure sintering
  97. Microwave technique applied on the hydrothermal synthesis and sintering of calcia stabilized zirconia nanoparticles
  98. Luminescence properties of cerium doped Y3Al5O12 nanopowders and nanoceramic
  99. Yttrium-Aluminum Garnet Synthesized in the Medium of Supercritical Fluids
  100. Advanced nanostructural zirconia ceramics for optical oxygen sensors
  101. Study of undoped and doped zirconia nanocrystals luminescence
  102. Producing metal oxide nanoparticles by the sonochemical, microwave and RAPET techniques.
  103. Doping of ZnO nanopowders with Mn, Ni and Cr In an ultrasound and microwave driver hydrothermal reaction
  104. Localization of rare-earth dopants in the lattice of nanocrystalline ZrO2 - EXAFS study
  105. Zirconia-based nanomaterials for oxygen sensor - generation, characterisation and optical properties
  106. Repair and regeneration of osteochondral defects in the articular joints
  107. Production of doped and non doped ceramics nanoparticles for optical applications
  108. Novel Methods for the Fabrication of Nanomaterials and Their Applications
  109. Synthesis, sintering and properties of doped nanocrystalline powders of zirconia, zinc oxide and yttrium aluminum garnet (YAG)
  110. Characterization of nanocrystalline ZrO2 doped with Rare-Earth elements synthesized via High Pressure Hydrothermal Method
  111. Particle Size Distribution of ZrO2:Pr3+- Influences of pH, High Power Ultrasound, Surfactant and Dopant Quantity
  112. Time-Resolved Luminescence Characteristics of Doped YAG and YAP Nanopowders
  113. Cathodoluminescence of Al doped ZnO nanopowders
  114. Morphology of Al doped Zinc Oxide Obtained using Hydrothermal and Vapour Condensation Methods
  115. Luminescence and structural properties of rare-earth doped YAG nanoceramics
  116. Optimization of conditions of preparation of YAG nanopowders for sintering of translucent ceramics
  117. The influence of ZrO2 containing 10% Eu 3+ on the polyurethane hard domain structure of nano-composites with luminescence properties
  118. Growth and properties of ytterbium doped KY(WO4)2 nanocomposites
  119. Investigations on the utilization of nano zinc oxides synthesized by hydrothermal method in rubber compounds
  120. Application of ultrasonically produced nano zerovalent iron for the degradation of chloroethenes
  121. Doping of ZnO nanopowders with Mn and Cr in an ultrasound and microwave driven hydrothermal reaction
  122. Investigations of phase composition and grain size distribution in Pr doped ZrO2
  123. Nasze wyniki, zdania i sposoby ich realizacji
  124. Morphology of Al doped zinc oxide obtained by the vapour condensation method
  125. The comparison of the properties of YAG obtained by coprecipitation and SCR method
  126. Synthesis of nanopowders at elevated pressure and their characterisation - summary of work of the WG-002
  127. Accuracy of XRD size measurements of nanocrystals
  128. Morphology of Al-Doped Zinc Oxide Obtained by the Vapour Condensation Methods
  129. SWOT analysis of various reactors used for synthesis of nanopwoders in our Working Group
  130. Preliminary research on zirconia based optical oxygen sensor
  131. Preparation and electrical properties of the BaTiO3 nanoceramics
  132. Current trends in the development of metallic nanomaterials: results of a study carried out within the EC project Nanoroad SME
  133. Microstructural Evolution in Mechanical Alloying and Hot Pressing of Aluminium and 316 Stainless Steel Powder Blend
  134. Time-resolved luminescence of nanostructured ZnO
  135. Prediction of degree of localisation of misfit dislocation cores in intercrystalline interfaces based on interfacial adhesion
  136. Kinetic and thermodynamic factors leading to dissolution of cementite in pearlitic steel subjected to severe plastic deformation under pressure
  137. Yttria stabilized zirconia nanocrystals luminescence
  138. Application of XRD diffraction methods for determination of grain size distribution in nanoparticles
  139. Optical investigations of RE-doped YAG nanoceramics
  140. Electrical properties of nanocrystalline ZrO2 at high-pressure
  141. High-pressure Induced Structural Decomposition of RE-doped YAG Nanoceramics
  142. Synthesis of Al-doped ZnO nanomaterials with controlled luminescence properties
  143. Combination of ECAP and Hydrostatic Extrusion for ultra-fine grain (UFG) microstructure generation in 99.98% nickel.
  144. Mechanical properties and deformation behaviour of ultra-fine grained nickel.
  145. Grain Size and Grain Size Distribution of Nanocrystalline Pr-doped Zirconia Powders Obtained in High Pressure Microwave Reactor
  146. Influence of synthesis conditions on the particles size and the morphology of zinc oxide nanopowders
  147. Measuring the Grain Size Distribution of Pr-doped Zirconia Nanopowders obtained by Microwave Driven Hydrothermal Synthesis
  148. Excellent mechanical properties of UFG metals and alloys, subjected to combination of severe plastic deformation methods.
  149. An FT-IR Spectroscopic Investigation of Hydroxide Groups in Nano-Crystals of ZrO2.
  150. Development of microstructure and mechanical properties in nickel deformed by hydrostatic extrusion
  151. Structural and luminescence properties of yttrium-aluminum garnet (YAG) nanoceramics
  152. Biocompatibility of thin films based on hydrothermal synthesized HAp
  153. Hybrid HAp- maleic anhydride copolymer nanocomposites obtained by in situ functionalisation
  154. Fabrication and electrical properties of Eu3+:BaTiO3 nanoceramics for SOFC
  155. Luminescence of ZrO2 nanocrystals
  156. Molecular impurities in the luminescent ZnO nanocrystals
  157. X-Ray investigations of the natural and artificial White Etching Layer
  158. Diffusion and diffusion induced defects in GaN
  159. Excellent Mechanical Properties of Nickel Processed by High Pressure Techniques
  160. Microwave driven hydrothermal synthesis of iron oxide - the effect of process parameter on the properties of the nanopowders
  161. Relaxation processes in ZrO2 at high pressures
  162. Luminescence of ZrO2 and ZnO nanocrystals
  163. Zinc oxide nanopowders obtained by the microwave-hydrothermal route
  164. Microwave driven hydrothermal synthesis of Pr-doped zirconia nanopowders
  165. New hydroxyapatite based nanomaterials for potential use in medical fields
  166. Correlation between high-pressure ZrO2 electrical properties and crystallite size
  167. Precise determination of full Grain Size Distribution from diffraction peak profile as a result of kinematical theory of diffraction for polidispersive nanomaterials
  168. Pressure Effect on Grain Boundary Diffusion in Al Bicrystals
  169. Microstructure of Surface Layers of Raiways After Heavy Exploatation
  170. Normal Grain Growth in 2-d Strips of Polycrystalline Aluminium Under High Pressure
  171. Reaction Diffusion in Metallic Systems under High Pressure
  172. High Pressure Effect on Grain Boundary Wetting in Aluminium Bicrystals
  173. Pressure Effect on Interface Energy, Diffusion and Reactions
  174. Microwave-Hydrothermal Synthesis of Nanostructured Pr-Doped Zirconia Powders
  175. The Strain Induced Cementite Dissolution in Carbon Steels-Experimental Facts and Theoretical Approach
  176. Effect of SPD Grain Refinement and Peculiarity of Structure and Mechanical Properties of UFG Ni
  177. Luminescense of Nanosize ZrO2
  178. Structure, Morphology and Luminescence Properties of Pr-doped Nanocrystalline ZrO2 Obtained by Hydrothermal Method
  179. Nanostructure Formation on the Surface of Railway Tracks and Wheelsets
  180. Hydrothermal Synthesis of Zinc Oxide Nanopowders with Microwaves Applications
  181. Microwave Driven Hydrothermal Synthesis of Iron Oxide Nanopowders
  182. Indentation Technique for Determination if Mechanical Behavior of Nanomaterials (Bulk and Coatings)

Presentation: Poster at Nano-Biotechnologia PL, by Aleksandra E. Kędzierska
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-07-23 12:09
Revised:   2012-09-16 09:32
Google
 
Web science24.com
© 1998-2022 pielaszek research, all rights reserved Powered by the Conference Engine