Novel method for bone tissue scaffold fabrication. Influence of composition chitosan/beta-TCP matrixes on mechanical and biological properties.

Martyna A. Kucharska 1Katarzyna Bocian 1Beata Butruk 1Tomasz Ciach 1Wojciech Swieszkowski 2Tomasz Brynk 2Marcin K. Heljak 2Małgorzata Szumieł-Lewandowska 3Agnieszka Mikulska 3

1. Warsaw University of Technology, Faculty of Chemical Engineering, Warynskiego 1, Warsaw 00-645, Poland
2. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
3. Medical University of Warsaw, Chałubińskiego 5, Warsaw 02-004, Poland


In this work authors present novel method of bone scaffolds fabrication. Matrixes are manufactured by chemical agglomeration of chitosan/β-TCP/collagen spheres and seem to be a promising bone tissue substitute. Fabrication process consists of the following steps: polymer based spheres extrusion, matrix formation by compression and spheres bonding with sodium tripolyphosphate (STPP) as cross-linking agent. Presented method allows to generate porous materials with controllable shape, pore size distribution and its interconnectivity. In this technique 3D scaffold porosity can be regulated by altering spheres diameter. Authors studied the influence of β-TCP, collagen and cross-linker concentrations on scaffold morphology, mechanical properties, enzymatic degradation rate (in presence of lysozyme), water absorption and osteoblast-like (MG-63) cells response. Influence of various chitosan molecular weights and length of STPP cross-linking on scaffold properties were also examined. Surface morphology and topography were evaluated by scanning electron microscope (SEM). Porosity and pores interconnectivity were observed via Micro-Computed Tomography (μCT) scanning. Mechanical tests showed that cross-linked chitosan performs compression characteristic (Young Modulus, maximum stress) similar to natural bone. Obtained matrices subjected to 10kN compression strength remain tough and not fractured. Cytotoxity evaluated by XTT assay shows that proposed composite materials don’t exhibit any toxic properties against human cells. Osteoblast-like cells adhesion and morphology were analyzed both by SEM and optical microscope.

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Presentation: Oral at E-MRS Fall Meeting 2008, Symposium L, by Martyna A. Kucharska
See On-line Journal of E-MRS Fall Meeting 2008

Submitted: 2008-05-26 18:54
Revised:   2009-06-07 00:48
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