Culture of osteoblasts on nanostructured titanium – preliminary observation

Halina Garbacz 1Małgorzata Lewandowska-Szumieł 2Edyta Wróbel Barbara D. Ostrowska 1Krzysztof J. Kurzydlowski 1

1. Warsaw University of Technology, Faculty of Materials Science and Engineering (InMat), Wołoska 141, Warszawa 02-507, Poland
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland


Commercial titanium of technical purity (c.p.) is an excellent implant material because of its high corrosion resistance and outstanding biocompatibility. It is known as not causing allergic reactions, preferred when infection is a risk. However, the applications of titanium are limited by relatively poor wear resistance and tensile strength. This stimulated interest in improving the mechanical properties of c.p. titanium, among others by hydrostatic extrusion which is a method of grain refinement by severe plastic deformation. Hydrostatic extrusion (HE) allows to obtain nanograins in bulk samples, which achieve strength similar to that of titanium alloys. This opens the new possibility for application of c.p. titanium in the biomedical devices. The present study reports the results obtained in the investigations of osteoblast culture on nanocrystalline titanium. The material examined in the study was c.p. extruded titanium with the mean grain size of 60 nm. HE titanium was examined by scanning and transmission microscopy (SEM/STEM Hitachi S 5500) and using a computer image analysis. The mechanical properties of the titanium were characterized using a HYSITRON Triboindenter. Titanium with a grain size of 20 μm and Ti6Al4V alloy were used as the reference materials. Human bone derived cells were observed in direct contact with investigated materials in vitro. Cell morphology and proliferation on nano-titanium was found to be comparable to those for micro-titanium and for Ti6Al4V alloy. This indicates, that nanocrystalline titanium obtained by hydrostatic extrusion may be taken into account as a material for bone reconstruction.


The study was support by the Ministry of Science and Higher Education.(grant 3 T08A 019 29) and by the Rector of Medical University of Warsaw (grant NZME/W2/07).

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: must be provided.


Related papers
  1. Financing of industry relevant projects in Poland
  2. Electrospun nanocomposites from PHBV for bone tissue engineering
  3. Nanotechnologie w gospodarce województwa podlaskiego
  4. Modeling of growth kinetics of SiC single crystal in PVT process
  5. Application of micro- and nanostructured biomaterials in regenerative medicine
  6. Core-shell P(LLA-CL) based electrospun nanofibers for peripheral nerve tissue engineering
  7. Surface modification of PCL scaffold changes protein adsorption
  8. TiO2 nanotubes based composite layers for biomedical applications
  9. Synthesis of highly biocompatible hydroxyapatite nanopowders
  10. Modification of epoxy resin properties by adding multi wall carbon nanotubes
  11. Możliwości zastosowania nanokrystalicznego tytanu w medycynie
  12. Możliwości badawcze Laboratorium Skaningowej Mikroskopii Elektronowej na WIM PW
  13. Otwarcie konferencji
  14. Perspektywy rozwoju nanomateriałów konstrukcyjnych i funkcjonalnych
  15. Zastosowania mikroskopii AFM dla farmacji i medycyny
  16. Wykorzystanie nanotechnologii w procesie odlewania precyzyjnego
  17. Oczyszczanie i modyfikacja nanomateriałów węglowych
  18. Modyfikacje polimerów stosowanych w lotnictwie za pomocą nanorurek węglowych
  19. Luminescent polyurethane nanocomposites: processing, structure and properties
  20. Micro Sensor for cell force measurement
  21. Preliminary studies on degradation of polylactides fibers
  22. Effect of grain size on reactive diffusion between Ti-Al
  23. TEM investigations of the nanostructure, multilayer CrN/Cr coatings obtained by vacuum-arc method
  24. Bulk  nanostructured  titanium  fabricated  by  hydrostatic  extrusion
  25. Composites PLLA/MWNTs produced by twin-screw microcompounder
  26. Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds
  27.  A  degradable and highly porous polyurethane scaffolds for bone tissue engineering
  28. Reduced activation ODS ferritic steel – recent development in high speed hot extrusion processing
  29. Investigation of the DLC and novel DLC-polymer hybrid coatings using FIB and SEM techniques
  30. A combination of microCT and FEM methods for development of a new scaffold for bone regeneration
  31. Properties of transparent elastomer nanocomposites (polyurethane/ ZrO2:Eu3+) as materials for optoelectronic application
  32. Investigation of fatique crack growth rate of Al 5484 ultrafine grained alloy after ECAP process
  33. Fire retardant polyurethane elastomers/aluminophosphorate nanocomposites for higher temperature application
  34. The influence of grain refinement on the corrosion mechanism of 7475 aluminium alloy
  35. Modelling the influence of the grain size distribution on the properties of nanometals
  36. Hybrid functional applied to the study of properties of narrow and wide band gap semiconductors
  37. Recrystallization and Grain Growth in Nano-structured Austenitic Stainless Steel under Electric Current Heating
  38. Effect of deformation and strain rate on diminishing of microstructure of aluminium alloys (AlCu4Zr0.5 and AlZn6Mg3CuZr)
  39. Grain refinement in CuCrZr by SPD processing
  40. Tailoring mechanical properties of nano-structured Eurofer 97 steel for fusion applications
  41. Microscopic and surface analytical characterization of TiO2 nanotubes produced by anodic oxidation and thermal treatment 
  42. A bit of physics in our understanding of the cell and tissue interaction with scaffolds
  43. Investigation of cells interactions with textile scaffolds for bone tissue enginnering
  44. Electrospun polymeric fibers for cartilage regeneration
  45. Biodegradable and bioabsorbable polymeric - ceramic composites for medical applications
  46. Modelling of plastic deformation of nano-polycrystalline materials
  47. Preliminary examinations of mechanical properties of DLC and novel DLC-polymer hybrid coatings for resurfacing hip prostheses
  48. Optimization of the polymeric scaffolds for bone tissue engineering fabricated by rapid prototyping method
  49. Bone tissue engineered product based on human bone derived cells and polyurethane scaffold
  50. Molecular statics simulation of nanoindentation on nanocrystalline copper
  51. Calcite-based material for tissue engineering ceramic scaffolds
  52. Microstructural and mechanical characterization of zirconia based composite
  53. Modeling of order-disorder phase transition in intermetallic compounds
  54. Optimization of polyurethane structure as the potential materials for bone tissue engineering applications
  55. Fabricationg polyurethane/calcium carbonate composite scaffold for bone tissue engineering
  56. Polyurethane nano-composites with luminescence properties for optics and optoelectronics applications.
  57. Nanomechanical measurements of thin dextran layers
  58. Tailoring mechanical properties by grain refinement and particle redistribution
  59. Stochastic Approach To The Changes In The Mechanical Properties Of Nano-Crystalline Materials Induced By Grain Growth
  60. Surface effect on atomic ordering in nano-layered L10 AB binaries: multiscale Monte Carlo Simulation
  61. Characterization of nanopowders
  62. Microstructure and mechanical properties of HE-treated titanium depending on its initial state
  63. Influence of hydrostatic extrusion parameters on the microstructure and mechanical properties of 6082 aluminium alloy
  64. The effect of grain size distribution on mechanical properties of nanometals
  65. Morphological and surface analytical characterization of chemically pretreated Ti surfaces intended for biomedical applications
  66. Electron microscopy studies of magnesium hydride
  67. Microstructure and mechanical properties of 7475 aluminium alloy processed by SPD methods
  68. Investigation of aqueous surface processes and corrosion mechanisms of Al-(Cu)-Fe-Cr quasicrystaline approximant
  69. Characterization of the effects of hydrostatic extrusion on structure and surface properties of 303 austenitic stainless steel
  70. The Effect of Hydrostatic Extrusion on the Corrosion Stability of Austenitic Stainless Steels
  71. The Effect of Hydrostatic Extrusion on Resistance of 316 Austenitic Stainless Steel to Pit Nucleation
  72. Nanomaterials in dental applications
  73. Nanostructure formation in austenitic stainless steel
  74. Evaluation of crystal structure and polytypism of SiC in microwave sintered diamond-SiC composite by TEM and HR-TEM
  75. Hydrogen induced changes in 316L stainless steel microstructure
  76. Statistical model of grain growth in polycrystalline nanomaterials
  77. Repair and regeneration of osteochondral defects in the articular joints
  78. Corrosion resistance of nanostructured titanium
  79. Effect of surface treatment on the microstructure of TA6V
  80. Predicting site occupation of Fe in B2 NiAl from ab initio computations
  81. Structure and properties of polyurethane/YAG:Tb3+ nano-composites with luminescence properties
  82. Structure and thermal properties of epoxy resins nano-composites with flame retardants
  83. The influence of ZrO2 containing 10% Eu 3+ on the polyurethane hard domain structure of nano-composites with luminescence properties
  84. An influence of boehmite modification on the dispersion in the polyurethane matrix
  85. Materials hardness estimation by simulation of the indentation process
  86. Stability of second phase particles during processing by hydrostatic extrusion
  87. The influence of nano - filler on microstructure homogeneity and selected properties of dental ceramic - polymer composites
  88. Grain Boundary Migration in Nanocrystalline Iron
  89. Thermal properties of L10-TiAl from first principle calculations
  90. Structural stability of zirconia ceramic in water and acid solutions
  91. The influence of the structure of metastable τ - phase on magnetic hysteretic properties of Мn55.5Аl44С0.5 alloy
  92. Morphology and chemical characterization of Ti surfaces modified for biomedical applications
  93. Evaluation of degradation degree of dental hybrid composite used as permanent fillings
  94. Optimization of particle reinforced lead-free solders
  95. Microstructural evaluation during particles addition to lead-free solders
  96. Thermal stability of hybrid polyurethane and modified boehmite
  97. Applications of quantitative image analysis to the description of the morphology of hybrid polyurea-nitryle-urethanes
  98. Improvement of wetting and bonding of SiO2 ceramics and urea-urethane elastomer
  99. A hybrid atomistic-continuum finite element modelling of locally disordered crystalline structure
  100. The role of the weak points in the corrosion resistance of hydrostatically extruded steel porducts
  101. The size effect in strength and fracture toughness of deformation origin submicro- and nanomaterials.
  102. The influence of hydrostatic extrusion on mechanical properties and tribological characteristics of an austenitic stainless steel
  103. Fabrication and micro-structure characterization of Al2O3/Ni-P composites with interpenetrating phases
  104. Hydrostatic extrusion of intermetallic Ti-Al layers
  107. Mechanical properties of aluminium processed by equal channel angular pressing
  108. Model for intermetallic phase and intermetallic compound solidification during diffusion soldering
  109. Texture Development in Model Al-Li Alloy Subjected to Severe Plastic Deformation
  110. The Influence of hydrostatic extrusion on microstructure of 6082 aluminum alloy
  111. Combination of ECAP and Hydrostatic Extrusion for ultra-fine grain (UFG) microstructure generation in 99.98% nickel.
  112. Microstructure refinement under high plastic strain rates in hydrostatic extrusion process
  113. Influence of severe plastic deformation on PLC effect in Al 5XXX alloy
  114. Mechanical properties and deformation behaviour of ultra-fine grained nickel.
  115. Formation of ultrafine gradient structure in hard magnetic alloy Fe-30%Cr-8%Co
  116. Sintering temperature effect on structure and properties of Al2O3/Ni-P composites with interpenetrating phases
  118. SEM investigations of tooth/ filling bonding
  119. Measuring the Grain Size Distribution of Pr-doped Zirconia Nanopowders obtained by Microwave Driven Hydrothermal Synthesis
  120. Development of microstructure and mechanical properties in nickel deformed by hydrostatic extrusion
  121. Nanometals for medical applications
  122. Effect of sever plastic deformation method on microstrucure and mechanical properties of Al and Cu
  123. The influence of twin bands density on microstructure of 316LVM austenitic stainless steel subjected to transverse rolling
  124. Microstructure evaluation in an Al-Li alloy processed by severe plastic deformation
  125. Polymer matrix composites with nanoparticles of TiC obtained by a sol-gel method
  126. Annealing temperature effect on residual stresses in 316L steel subjected to HPT process
  129. Cells and scaffolds for tissue engineering - what is needed for success?
  131. Modelling the interaction of crack tip with nanostructure, which creating by deformation.
  133. Porous polymeric scaffolds for bone regeneration
  134. Effect of diisocyanate chemical structure on biocompatibility of segmented polyurethanes
  136. Atomic-scale Ab-initio computations of the B2-RuAl
  137. Ab-initio calculations of elastic properties Ni3Al and TiAl under pressure
  138. Poly(epsilon-caprolactone)- and poly(methyl methacrylate)-based scaffolds in human bone-derived cell cultures in vitro
  139. Mechanical characteristics of polymeric support as an important factor in osteoblast culture in vitro
  140. Alginate hydrogel-candidate support for cell transplantation - preliminary observation in human chondrocyte culture
  141. Modelling of the Influence of the Grain Size Distribution on the Grain Growth in Nanocrystals
  142. Omentum major as potential source of osteogenic cells for tissue engineering (preliminary report)
  143. Ductility of Nanocrystalline Materials Processed by Severe Plastic Deformation
  144. Structural-strength aspects of glass/metallic composite based on Co-Cr-Mo implantation alloy
  145. Microstructure and mechanical properties of binary Al-Li alloys processed by ECAP
  146. Microstructure of the ceramic - polymer composites for dental applications
  147. Effect of Non-conventional Large Deformations on Microstructure and Properties of Metals
  148. Excellent Mechanical Properties of Nickel Processed by High Pressure Techniques
  149. Morphology and properties of oxide films formed on the nanocrystalline 316L austenite steel
  150. Al2O3-Fe functionally graded materials fabricated under magnetic field
  151. Differentiation of cells from adipose tissue into osteoblasts in the presence of biomaterials in vitro (preliminary report)
  152. Alternative methods bring a new input in biocompatibility testing
  153. Professor CZOCHRALSKI - Distinguished Scientist and Inventor, Founder of the Modern Materials Science
  154. The influence of temperature and pressure on possibility of obtaining Al2O3/Ni-P nanocomposite through hot pressing process.
  155. Mechanical properties nanostructured iron obtained by different methods of Severe Plastic Deformation
  156. Strain localization in nanocrystalline iron after Severe Plastic Deformation
  157. Non-elastic behaviours of high-porosity ceramics and ceramic-polymer composites
  158. The Superplasticity of Hard Magnetic Alloy Fe-23wt.%Co-30%Cr with Submicrocrystalline Structure
  159. Microstructure of Ni-P thin layers obtained by a chemical method
  160. Hydrostatic extrusion and nanostructure formation in an aluminium alloy
  161. Quantitative Description of Microstructure of Nano-materials
  162. The Structure and Mechanical Properties of Low Carbon Low Alloy Steel Subjected to Severe Plastic Deformation
  163. Studies of Ni-P Thin Films by Electroles Deposition Method
  164. Stereology of Nanomaterials
  165. Targeted Research Project: Metallic, Ceramic and Organic Nanomaterials: Processing - Structure - Properties - Applications
  166. Materials Research in Poland

Presentation: Poster at E-MRS Fall Meeting 2008, Symposium L, by Barbara D. Ostrowska
See On-line Journal of E-MRS Fall Meeting 2008

Submitted: 2008-07-09 11:15
Revised:   2009-06-07 00:48
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine