Electrochemical communication between viable bacterial cells and flexible redox polymers

Kamrul Hasan 1Kamil Górecki 2Cecilia Hägerhäll 2Lo Gorton 2

1. Lund University, Department of Analytical Chemistry and Biochemistry, Lund 22100, Sweden
2. Lund University, Biochemistry, Getingevagen 60, Lund SE-22100, Sweden


Recently we have shown that bacterial cells can be electrochemically ”wired” to electrodes with flexible redox polymers e.g., poly(1-vinylimidazole)12-[Os(4,4’-dimethyl-2,2’-dipyridyl)2Cl2]2+/3+ and poly(vinylpyridine) [Os(N,N’-dimethyl-2,2’-biimidazole)3]2+/3+. Our initial studies1 were made with the simple Gram-negative Gluconobacter oxydans, where we addressed redox enzymes from the cytoplasmic membrane yielding response for glucose, fructose, ethanol and glycerol. Later focus was on more complex Gram-negative Pseudomonas putida and P. fluorescens,2,3 where response currents were obtained for substrates metabolised in the cytoplasmic membrane (glucose) and in the cytosol (phenol). Recently introduction of a cytochrome to the cytoplasmic membrane of E. coli facilitated the communication between E. coli cells and the redox polymers.4 In Gram-positive B. subtili 5 strain which overproduces complex II, current resonse has been improved several times although it was expected to be more difficult for the thick cell wall to permeate by rodox polymer. Another recent work that supports such a theory is a paper by Marshall and May6, who show that Gram-positive Thermincola ferriacetica strain Z-0001 readily can grow onto a graphite electrode and exhibit direct e- transfer communication. Currently we are investigating Rhodobacter capsulatus, one of the most metabolic versatile bacteria in the nature, grown heterotrophically and successfully wired with poly (1-vinylimidazole) 12-[Os(4,4’-dimethyl-2,2’bipyridyl)2Cl]2+/+, E°’= 200 mV vs. SCE7 in both batch and flow mode. More experiments are going on to establish the communication between phtoheterotrophicaly grown cells and the redox osmium polymers followed by electrode by using light as a energy source instead of any organic substrate.



[1] I. Vostiar, et al., Electrochem. Commun. 6 (2004) 621-626.

[2] S. Timur, et al., Bioelectrochemistry 71 (2007) 38-45.

[3] S. Timur, et al., Electrochem. Commun. 9 (2007) 1810-1815.

[4] S. Alferov, et al. Electrochim. Acta 54 (2009) 4979-4984.

[5] V. Coman, et al, J. Am. Chem. Soc., 131 (2009) 16171-16176.

[6] C. W. Marshall, H. D. May, Energy Environ. Sci. 2 (2009) 699-705.

[7] M. N. Zafar et al.,Bioelectrochemistry 80(2001)38-42

Auxiliary resources (full texts, presentations, posters, etc.)
  1. POSTER: Electrochemical communication between viable bacterial cells and flexible redox polymers, Zip archive data, at least v2.0 to extract, 0MB

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/24882 must be provided.


Related papers
  1. Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells
  2. Deglycosylation of glucose oxidase by PNGase F
  3. Influence of metal cations on the turnover rate of cellobiose dehydrogenase
  4. Electron transfer studies with different sugar oxidizing enzymes and osmium polymers to improve the current density
  5. Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications
  6. Direct electrochemistry of cellobiose dehydrogenase for applications in the third-generation biosensor and biofuel cell
  7. Electrochemical Communication between Viable Bacterial Cells and Flexible Redox Polymers
  8. Biosensing Applications Of Engineered Pyranose 2-oxidases Wired With Osmium Polymers
  9. Anode and cathode reactions for biofuel cells based on direct electron transfer reactions between biological components and electrodes
  10. Increasing Biosensor Sensitivity by Length Fractionated Single Walled Carbon Nanotubes
  11. Electrical Wiring of Living Bacillus subtilis Cells Using Flexible Osmium-Redox Polymers
  12. Some electrochemical properties of laccase immobilised on the Au, IrOx, or C60-Pd polymer electrode supports
  13. Oxygen electroreduction by fungal laccases - combination of electrochemical and spectral data
  14. Wiring of whole living bacteria with osmium-redox polymers
  15. The electrochemistry of a his-tagged microperoxidase assembled onto gold electrodes
  16. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes

Presentation: Poster at SMCBS'2011 International Workshop, by Kamrul Hasan
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-08-24 17:14
Revised:   2011-08-31 17:33
Web science24.com
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine