Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications

Magnus Falk 1Xiaoju Wang 2Roberto Ortiz 3Lo Gorton 3Roland Ludwig 4Sergey Shleev 1

1. Malmö Univerity Health and Society, None, Malmö 20506, Sweden
2. Åbo Akademi University, Turku 20500, Finland
3. Department of Analytical Chemistry and Biochemistry, Lund University,, P.O Box 124,, Lund SE-22100, Sweden
4. Division of Food Biotechnology, Department of Food Sciences and Technology,, BOKU-University of Natural Recources and Applied Life Sciences Vienna, Muthgasse 18,, Wien A-1190, Austria

Abstract

We report on the fabrication and characterisation of mediator-less sugar and oxygen sensitive biodevices based on three-dimensonal gold nanoparticle-modified electrodes with immobilised sugar oxidising and oxygen reducing enzymes. To create sugar and oxygen sensitive biodevices, Corynascus thermophilus cellobiose dehydrogenase (CtCDH) and Myrothecium verrucaria bilirubin oxidase (MvBOx) were used, respectively. For CtCDH modified bioelectrodes maximal current densities of 28 µA cm-2 and 40 µA cm-2 could be obtained in the presence of 120 mM glucose and 5 mM lactose, respectively. It was shown that bioelectrocatalytic oxidation of sugars on CtCDH-based electrodes was limited by the  activity of the enzyme. Contrary, in the case of MvBOx modified biodevices, a maximal current density equal to 110 µA cm-2 was obtained in air saturated solution due to mass transfer limitation.

By connecting the electrodes together a membrane- and mediator-less sugar/oxygen biofuel cell (BFC) was created operating in buffers and human physiological liquids. The following characteristics of the mediator-, separator- and membrane-less, miniature BFC, were obtained: open-circuit voltages of 0.68 and 0.65 V, maximum power densities of 15 mW cm-2 and 3 mW cm-2 at 0.52 V and 0.45 V of cell voltage, in phosphate buffer and human blood, respectively. The estimated half-lifes of biodevices were measured to be 24 h and 8 h in sugar-containing buffers and human physiological liquids, respectively. Thus, a mediatorless sugar/oxygen BFC with significantly improved basic characteristics compared to previously designed biodevices1,2 could be constructed because of the usage of three-dimensional gold nanoparticle-modified electrodes.

1. V. Coman, R. Ludwig, W. Harreither, D. Haltrich, L. Gorton, T. Ruzgas, and S. Shleev. (2010) A direct electron transfer-based glucose/oxygen biofuel cell operating in human serum. Fuel Cells. 10(1), 9-16.2. V. Coman, C. Vaz-Domínguez, R. Ludwig, W. Harreither, D. Haltrich, A. L. De Lacey, T. Ruzgas, L. Gorton and S. Shleev. (2008) A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell. Phys. Chem. Chem. Phys. 10(40), 6093–6096.

Acknowledgements: The authors thank Amano Enzyme Inc. for Amano 3 preparation of M. verrucaria bilirubin oxidase. The authors would like to also thank Petri Gudmundsson and Karin Nilsson (Malmö University, Sweden) for their kind help with blood collection. Ms. Wang would like to thank the Finnish Graduate School of Chemical Engineering (GSCE) for the financial support to her PhD study and the scholarship for her research visit at Lund University in 2010. The work has been supported financially by the European Commission (FP7 project NMP4-SL-2009-229255) and the Swedish Research Council (projects 2007-4124, 2009-3266 and 2010-5031).

 

Auxiliary resources (full texts, presentations, posters, etc.)
  1. PRESENTATION: Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications, Microsoft Office Document, 0MB
  • Legal notice:

    The above materials are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced, stored in or introduced into a retrieval system, or transmitted in any form or by any means (electronic, mechanical, photocopying, recording or otherwise), or for any purpose, without the express written permission of Pielaszek Research Corporation, the Publisher.
    Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations. In such a case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/24908 must be provided.

 

Related papers
  1. Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells
  2. Deglycosylation of glucose oxidase by PNGase F
  3. Potentially implantable bioelectronic devices for biosensing and biofuel cell applications
  4. Influence of metal cations on the turnover rate of cellobiose dehydrogenase
  5. Electron transfer studies with different sugar oxidizing enzymes and osmium polymers to improve the current density
  6. Electrochemical communication between viable bacterial cells and flexible redox polymers
  7. Direct electrochemistry of cellobiose dehydrogenase for applications in the third-generation biosensor and biofuel cell
  8. Electrochemical Communication between Viable Bacterial Cells and Flexible Redox Polymers
  9. Biosensing Applications Of Engineered Pyranose 2-oxidases Wired With Osmium Polymers
  10. Laccase-redoxpolymer cathodes for biofuel cells. Evaluation using an electrochemical robotic system.
  11. Enzymatic fuel cells
  12. Anode and cathode reactions for biofuel cells based on direct electron transfer reactions between biological components and electrodes
  13. Increasing Biosensor Sensitivity by Length Fractionated Single Walled Carbon Nanotubes
  14. Electrical Wiring of Living Bacillus subtilis Cells Using Flexible Osmium-Redox Polymers
  15. Some electrochemical properties of laccase immobilised on the Au, IrOx, or C60-Pd polymer electrode supports
  16. Amperometric determination of formaldehyde by the use of the yeast Hansenula polymorpha as a bioselective element
  17. Oxygen electroreduction by fungal laccases - combination of electrochemical and spectral data
  18. Wiring of whole living bacteria with osmium-redox polymers
  19. The electrochemistry of a his-tagged microperoxidase assembled onto gold electrodes
  20. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes

Presentation: Short communication at SMCBS'2011 International Workshop, by Magnus Falk
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-08-29 17:25
Revised:   2011-08-29 17:39