Electrical Wiring of Living Bacillus subtilis Cells Using Flexible Osmium-Redox Polymers

Vasile Coman 1Cecilia Hägerhäll 2Lo Gorton 1

1. Lund University, Department of Analytical Chemistry, Lund SE-221 00, Sweden
2. Lund University, Biochemistry, Getingevagen 60, Lund SE-22100, Sweden

Abstract

Artificial mediators offer the possibility for an enhanced electron transfer between microbial cells and electrodes. The important role of these electron shuttles in whole cell biosensors and microbial fuel cells is to replace the natural electron acceptor (oxygen in case of aerobic bacteria, Fe(III) oxides/complexes in case of anaerobic organisms), thus preventing the problem of limiting concentrations. The polymeric mediators proved to exhibit efficient electron shuttling properties for multiple layers of microbial cells. They promote a stable binding on the electrode surface; therefore the problem of releasing possible human-toxic compounds in the environment is reduced. In the present study the application of two different flexible osmium redox polymers; poly(1-vinylimidazole)12-[Os-(4,4′-dimethyl-2,2′-di'pyridyl)2Cl2]2+/+ (osmium redox polymer I) and poly(vinylpyridine)-[Os-(N,N′-methylated-2,2′-biimidazole)3]2+/3+ (osmium redox polymer II) were investigated for efficient electrical wiring of gram-positive bacteria Bacillus subtilis. Their efficiency in wiring gram-negative bacteria was previously investigated [1,2]. The two Os-polymers differ in redox potential and the length of the side chains, where the Os2+/3+-functionalities are located. The analytical characteristics of the microbial sensors were evaluated for determination of succinate, fumarate and glucose as substrates in both batch analysis and flow analysis mode using both gold and graphite electrodes. The influence of oxygen presence in the buffer on the current response was evaluated. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide).

[1] I. Vostiar, E. E. Ferapontova, L. Gorton, Electrochem. Commun. 6 (2004) 621.

[2] S. Timur, B. Haghighi, J. Tkac, N. Pazarlioglu, A. Telefoncu, L. Gorton, Bioelectrochemistry 71 (2007) in press.

 

Related papers
  1. Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells
  2. Deglycosylation of glucose oxidase by PNGase F
  3. Influence of metal cations on the turnover rate of cellobiose dehydrogenase
  4. Electron transfer studies with different sugar oxidizing enzymes and osmium polymers to improve the current density
  5. Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications
  6. Electrochemical communication between viable bacterial cells and flexible redox polymers
  7. Direct electrochemistry of cellobiose dehydrogenase for applications in the third-generation biosensor and biofuel cell
  8. Electrochemical Communication between Viable Bacterial Cells and Flexible Redox Polymers
  9. Biosensing Applications Of Engineered Pyranose 2-oxidases Wired With Osmium Polymers
  10. Anode and cathode reactions for biofuel cells based on direct electron transfer reactions between biological components and electrodes
  11. Increasing Biosensor Sensitivity by Length Fractionated Single Walled Carbon Nanotubes
  12. Some electrochemical properties of laccase immobilised on the Au, IrOx, or C60-Pd polymer electrode supports
  13. Oxygen electroreduction by fungal laccases - combination of electrochemical and spectral data
  14. Wiring of whole living bacteria with osmium-redox polymers
  15. The electrochemistry of a his-tagged microperoxidase assembled onto gold electrodes
  16. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes

Presentation: Poster at SMCBS'2007 International Workshop, by Vasile Coman
See On-line Journal of SMCBS'2007 International Workshop

Submitted: 2007-08-28 11:11
Revised:   2009-06-07 00:44