Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells

Roberto Ortiz 1Hirotoshi Matsumura 1,2Kiyohiko Igarashi 2Federico Tasca 1Roland Ludwig 3Lo Gorton 1

1. Department of Analytical Chemistry and Biochemistry, Lund University,, P.O Box 124,, Lund SE-22100, Sweden
2. The University of Tokyo, Dept. Biomat. Sci., Grad. Sch. of Agri. and Life Sci., Bunkyo-ku, Tokyo 113-8657, Japan
3. BOKU-University of Natural Resources and Life Sciences, Institute of Food Technology, Muthgasse 18, Vienna 1190, Austria

Cellobiose dehydrogenase (CDH) is an extracellular highly glycosylated two domain redox enzyme, which is one of the very few redox enzymes that shows direct electron transfer (DET) properties with electrodes. The catalytically active domain contains flavin adenine dinucleotide (FAD) and the other contains heme b. For the native glycosylated enzyme only the heme domain shows DET properties reflected by that the redox conversion of the heme group is shown in cyclic voltammetry (CV). For the native enzyme DET of the FAD domain is not show. Recently we initiated studies of deglycolysated CDH aiming at obtaining more efficient DET properties in line with previous reports for horseradish peroxidase [1,2] and glucose oxidase [3]. Ceriporiopsis subvermispora (Cs) and Phanerochaete chrysosporium (Pc) CDH were used in this study. When deglycosylated CDH was used instead CV revealed signals from both the heme and FAD. However, in the presence of substrate catalytic currents in CV only emanate at potentials close that of the heme for both CDHs.

When investigated adsorbed on graphite electrodes CsCDH (16% glycosylation) its deglycosylated equivalent shows at least 3 times as high catalytic currents as its glycosylated counterpart. PcCDH (9% glycosylation) shows twice higher currents for its deglycosylated form. A similar behavior is observed on thiol modified gold electrodes for CsCDH but not for PcCDH. In this last case both the glycosylated and deglycosylated variants show equally high catalytic currents. The improvement in current response on graphite is due to a higher amount of deglycosylated enzyme immobilized on the graphite electrode. The basic bioelectrochemistry as well as the bioelectrocatalytic properties will be shown as well as applications of CDH modified electrodes as 3rd generation biosensors and as bioanodes in biofuel cells.


[1] G. Presnova, V. Grigorenko, A. Egorov, T. Ruzgas, A. Lindgren, L. Gorton, T. Börchers, Faraday Discus., 116 (2000) 281-289.

[2] E. E. Ferapontova, V. G. Grigorenko, A. M. Egorov, T. Börchers, T. Ruzgas, L. Gorton, Biosens. Bioelectron., 16 (2001) 147 - 157.

[3] O. Courjean, F. Gao, N. Mano, Angew. Chem. Int. Ed., 2009, 48, 5897 –5899.


This work was financially supported by the European Commission, “3D-Bionanodevice” NMP4-SL-2009-229255.

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/24940 must be provided.


Related papers
  1. Deglycosylation of glucose oxidase by PNGase F
  2. Influence of metal cations on the turnover rate of cellobiose dehydrogenase
  3. Electron transfer studies with different sugar oxidizing enzymes and osmium polymers to improve the current density
  4. Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications
  5. Electrochemical communication between viable bacterial cells and flexible redox polymers
  6. Direct electrochemistry of cellobiose dehydrogenase for applications in the third-generation biosensor and biofuel cell
  7. Electrochemical Communication between Viable Bacterial Cells and Flexible Redox Polymers
  8. Biosensing Applications Of Engineered Pyranose 2-oxidases Wired With Osmium Polymers
  9. Anode and cathode reactions for biofuel cells based on direct electron transfer reactions between biological components and electrodes
  10. Increasing Biosensor Sensitivity by Length Fractionated Single Walled Carbon Nanotubes
  11. Electrical Wiring of Living Bacillus subtilis Cells Using Flexible Osmium-Redox Polymers
  12. Some electrochemical properties of laccase immobilised on the Au, IrOx, or C60-Pd polymer electrode supports
  13. Oxygen electroreduction by fungal laccases - combination of electrochemical and spectral data
  14. Wiring of whole living bacteria with osmium-redox polymers
  15. The electrochemistry of a his-tagged microperoxidase assembled onto gold electrodes
  16. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes

Presentation: Poster at SMCBS'2011 International Workshop, by Lo Gorton
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-08-31 17:42
Revised:   2011-08-31 17:43
Web science24.com
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine