Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells
|Roberto Ortiz 1, Hirotoshi Matsumura 1,2, Kiyohiko Igarashi 2, Federico Tasca 1, Roland Ludwig 3, Lo Gorton 1|
1. Department of Analytical Chemistry and Biochemistry, Lund University,, P.O Box 124,, Lund SE-22100, Sweden
When investigated adsorbed on graphite electrodes CsCDH (16% glycosylation) its deglycosylated equivalent shows at least 3 times as high catalytic currents as its glycosylated counterpart. PcCDH (9% glycosylation) shows twice higher currents for its deglycosylated form. A similar behavior is observed on thiol modified gold electrodes for CsCDH but not for PcCDH. In this last case both the glycosylated and deglycosylated variants show equally high catalytic currents. The improvement in current response on graphite is due to a higher amount of deglycosylated enzyme immobilized on the graphite electrode. The basic bioelectrochemistry as well as the bioelectrocatalytic properties will be shown as well as applications of CDH modified electrodes as 3rd generation biosensors and as bioanodes in biofuel cells.
 G. Presnova, V. Grigorenko, A. Egorov, T. Ruzgas, A. Lindgren, L. Gorton, T. Börchers, Faraday Discus., 116 (2000) 281-289.
 E. E. Ferapontova, V. G. Grigorenko, A. M. Egorov, T. Börchers, T. Ruzgas, L. Gorton, Biosens. Bioelectron., 16 (2001) 147 - 157.
 O. Courjean, F. Gao, N. Mano, Angew. Chem. Int. Ed., 2009, 48, 5897 –5899.
This work was financially supported by the European Commission, “3D-Bionanodevice” NMP4-SL-2009-229255.
Presentation: Poster at SMCBS'2011 International Workshop, by Lo Gorton
See On-line Journal of SMCBS'2011 International Workshop
Submitted: 2011-08-31 17:42 Revised: 2011-08-31 17:43
|© 1998-2021 pielaszek research, all rights reserved||Powered by the Conference Engine|