Novel nanostructural photosensitizers for photodynamic therapy

Kinga Tataruch 1Aleksandra Rusin 2Mariusz Kępczyński 3Zdzisław Krawczyk 2Maria Nowakowska 3

1. Cracow University of Economics, Faculty of Commodity Science, Rakowicka 27, Kraków 31-510, Poland
2. Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice 44-100, Poland
3. Jagiellonian University, Faculty of Chemistry, Ingardena 3, Kraków 30-060, Poland

Abstract

      Photodynamic therapy is currently used as a popular form of photochemotherapy for a wide variety of clinical applications. This treatment requires the presence of a drug, called photosensitizer, activating light of a specific wavelength and molecular oxygen to produce a localized damage in tumor tissues and/or their vasculature. Many efforts have been undertaken to develop new agents with a high efficacy for photodynamic treatment of cancer and some other diseases. Porphyrins and their derivatives are studied as potential sensitizers because of their high anti-cancer efficiency. The factors limiting the use of porphyrins in PDT are their poor solubility in water, low chemical purity and high dark toxicity [1].

     This paper presents the results of our studies on the development of the efficient nanoformulations of porphyrin for PDT. In the first step three PEG-functionalized porphyrins (p-THPP-PEG350, p-THPP-PEG2000 and p-THPP-PEG5000) were synthesized by covalent attachment of PEG chains of various molecular weights (350, 2000 and 5000 Da) to commercially available 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP). To improve their ability to penetrate the hydrophobic cell membrane, the conjugates were solubilized in liposome vesicles.

     The usefulness of novel hybrid systems as photosensitizers in photodynamic therapy was tested in vitro. Dark and photo cytotoxicity of these photosensitizers delivered in solution or embedded in liposomes were evaluated on two cell lines: HCT 116 and DU 145 and compared with these treated with free p-THPP. The kinetics of cellular uptake and the intracellular co-localization of p-THPP-PEG2000 formulations (which exhibit the most significant photodynamic activity) were studied by means of flow cytometric analysis and confocal laser scanning microscopy with the application of properly chosen fluorescence markers. Additionally, the time course and the mechanism of cell death after PDT were determined [2].

[1] Kessel D. Photodiagnosis and Photodynamic Therapy 2004, 1,3.

[2] Nawalany K. et al. International Journal of Pharmeceutics 2012, 430, 129-140.

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/28297 must be provided.

 

Related papers
  1. Nano/micro drug carriers
  2. Unsaturated saccharides as a useful chiral synthons and their application in synthesis  of polyphenol glycoconjugates - potential antiproliferative agents
  3. Synthesis and biological activity of synthetic β-D-glucose and β-D-galactose conjugates with various acyclic nucleosides
  4. Evaluation of cytoxicity of glycosyl uridine derivatives
  5. Prediction of intestinal absorption and metabolism of biologically active genistein derivatives
  6. Studies on the inhibition mechanism of the human coronavirus NL63 replication by polycations
  7. Polisacharydowo-białkowe membrany do rekonstrukcji rogówki
  8. Protoporphyrine IX and its PEI derivatives as possible photosensitizer in antibacterial photodynamic therapy
  9. Albumin-HMchitosanGTMAC-based nanoparticles as potential controlled release delivery systems of curcumin
  10. Selective adsorbents of adenosine-5'-triphosphate
  11. Novel polymeric inhibitors of human coronavirus NL63
  12. Superparamagnetyczne nanocząstki tlenku żelaza opłaszczane pochodnymi chitozanu – nowe kontrasty do MRI
  13. Polymeric Nanocapsules for Controlled Delivery of Lipophilic Drugs
  14. Comparison of biological activity and stability of selected C-glycosidic and O-glycosidic genistein derivatives
  15. The role of a sugar moiety in new genitein derivatives affecting proliferation and apoptosis of cancer cells
  16. Synthesis and in vitro screening of antiproliferative activity of C-glycosides, the derivatives of genistein and daidzein in selected cancer cell lines
  17. Dextran-based materials reducing anticoagulant activity of heparin in animal models in vitro and in vivo
  18. Preliminary studies on a new way of nucleic acids extraction using hydrogel microsphere
  19. Properties of DODAB/oleyl alcohol and DODAB/cholesterol monolayers and bilayers
  20. Antiproliferative activity of novel synthetic genistein glycoside derivatives
  21. Synthesis and physicochemical properties of cationic chitosan derivative for heparin complexation
  22. Cationic chitosan derivative for heparin reversal - in vitro and in vivo studies in rats

Presentation: Poster at Nano-Biotechnologia PL, by Kinga Tataruch
See On-line Journal of Nano-Biotechnologia PL

Submitted: 2012-06-29 15:03
Revised:   2012-06-29 15:03
Google
 
Web science24.com
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine