The aim of this work is determination of cytotoxicity of uridine derivatives. We indicated toxicity of the tested compounds in HCT 116 and DU 145 cell lines with use of MTT assay. Cells were plated in 96-well plate at the density 2*103 (HCT 116) or 3*103 (HCT 116) cells per well and grown for 24h. Then, the cells were treated by the tested compounds at growing series of concentrations: 0.01, 0.1, 0.5, 1.0, 5.0, 10.0, 25.0, 50.0 and 100μM for 72h. After 3h incubation with MTT substrate (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide) the absorbance of samples was measured spectrophotometrically with a microplate reader at 570nm wavelength. 

Our result indicate that described compounds did not affect proliferation of the tested cell lines.

Acknowledgement:

Research studies part-financed by the European Union within the European Regional Development Fund (POIG.01.01.02-14-102/09). Roman Komor received a scholarship under the project DoktoRIS - Scholarship Program for Innovative Silesia.

References:

[1] C. Breton; L. Šnajdrová; C. Jeanneau, J. Koca, A. Imberty, Glycobiology, 2006, 16, 29R–37R

[2] K. Hosoguchi, T. Maeda,J. Furukawa, Y. Shinohara, H. Hinou, M. Sekiguchi, H. Togame, H. Takemoto, H. Kondo, S.-I. Nishimura, J. Med. Chem. 2010, 53, 5607–5619  

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