Albumin-HMchitosanGTMAC-based nanoparticles as potential controlled release delivery systems of curcumin
|Dorota Bielska 1, Katarzyna Krzysztofek , Anna Karewicz , Maria Nowakowska|
1. Jagiellonian University, Faculty of Chemistry, Ingardena 3, Kraków 30-060, Poland
Curcumin is a natural polyphenol derived from a traditional herbal remedy. It shows the promising anti-inflammatory, anti-cancer and anti-oxidant activities. It is, however, only sparingly soluble in water and therefore its bioavailability is very low.As a solution to that problem we propose a new type of hybrid nanocarrier for effective curcumin. The carrier is based on bovine serum albumin (BSA) and N-dodecyl-N-glycidyltrimethylammonium-chitosan (HMchitosanGTMAC). Albumin is known for its extraordinary ligand binding capacity and non-covalent reversible binding characteristics. Chitosan is a cationic, biocompatible polymer. Stable aqueous dispersion of bovine serum albumin (BSA) nanoparticles NPs containing curcumin was obtained by a coacervation process followed by electrostaticly driven adsorption of cationic N-dodecyl-N-glycidyltrimethylammonium-chitosan (HMchitosanGTMAC) on the NP surfaces. Variuos concentrations of HMchitGTMAC were used for coating of BSA NPs to stabilize the colloidal system and to control the release of curcumin. The physicochemical properties of the resulting objects were then studied. The size and surface potential of the nanoparticles were evaluated using dynamic light scattering (DLS) measurements. The morphology of the unloaded and curcumin-loaded nanocarriers was evaluated by Atomic Force Microscopy (AFM) and by Scanning Electron Microscopy (SEM). The binding constant (Ka) of curcumin to bovine serum albumin (BSA) and curcumin release profiles were determined. The high Ka value and plausible release profiles suggest that our novel delivery system, based on albumin, may act as an effective carrier for curcumin.
Acknowledgements: Project operated within the Foundation for Polish Science Team Programme co-financed by the EU European Regional Development Fund, PolyMed, TEAM/2008-2/6.
Presentation: Poster at Nano-Biotechnologia PL, by Dorota Bielska
See On-line Journal of Nano-Biotechnologia PL
Submitted: 2012-06-29 10:52 Revised: 2012-06-29 10:54
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