Preliminary studies on a new way of nucleic acids extraction using hydrogel microsphere
|Kamil K. Kamiński , Michał Szuwarzyński , Krzysztof Szczubiałka , Maria Nowakowska|
Jagiellonian University, Faculty of Chemistry, Ingardena 3, Kraków 30-060, Poland
In many areas of modern medicine and molecular biology the isolation of a nucleic acid from the biological material is a key process. It is a preliminary step for many procedures without which the study on DNA or RNA would not be possible . Hence there is a need to develop better methods to perform these procedures. The most serious problem with the techniques used today is the use of toxic solvents are often harmful to the environment and the laboratory personnel performing isolation. This work presents the results of the studies on the use of polymeric hydrogels based on fully biocompatible and biodegradable biomaterials for this purpose.
Figure 1. Atomic force microscope picture of the studied microspheres
The average size of the obtained structures, and size distribution was determined. The ability of microspheres to bind DNA and other anionic biopolymers was studied. Also, the the possibility of the interaction of the microspheres with bovine serum albumin was examined.
Acknowledgement: The project was operated within the Fundation for Polish Science Team Programme (TEAM/2008-2/6) and Ventures Programme (Ventures/2009-4/4) cofinanced by EU European Regional Development Fund
Presentation: Poster at VIII Multidyscyplinarna Konferencja Nauki o Leku, by Kamil K. Kamiński
See On-line Journal of VIII Multidyscyplinarna Konferencja Nauki o Leku
Submitted: 2012-02-22 23:54 Revised: 2012-02-23 00:01
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