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The transcripional activity of genes encoding MMPs and TIMPs in breast cancer cells treated by genistein and in normal cancer-associated fibroblasts - in vitro studies

Małgorzata T. Latocha 1Justyna Płonka 1Dariusz Kuśmierz 1Magdalena E. Jurzak 2Renata M. Polaniak 3Ada Nowosad 1

1. Department of Cell Biology, Medical University of Silesia (SUM), Jedności 8, Sosnowiec 41-200, Poland
2. Department of Cosmetology Medical University of Silesia (SUM), Kasztanowa 3, Sosnowiec 40-055, Poland
3. Department of General Biochemistry, Medical University of Silesia (SUM), Jordana 19, Zabrze 41-808, Poland


Breast cancer is one of the most common cancers among women. Thus, the scientists are still looking for the new efficient therapeutic methods of treatment.

Phytoestrogens are currently in the center of  attention because of their therapeutic and preventive proprieties. Genistein is a naturally occurring plant compound from the group of phytoestrogens which possess many therapeutic activities. Its anticancer effects occur at multiple levels, including cell cycle regulation, induction of programmed cell death and inhibition of angiogenesis and metastasis.

The aim of this study was to examine the impact of genistein on genes involved in MMPs and TIMPs synthesis in breast cancer cells and normal fibroblasts stimulated by medium derived from T-47 D cultures. We used slightly invasive hormone-dependent T-47D (ATCC) breast cancer cells, subtype Luminal A and normal fibroblasts HFF-1 (ATCC) isolated from skin. Quantitation of the genes encoding MMP-1, -2, -3 -9, -13, -14, -15 and tissue inhibitors of metalloproteinase : TIMP-1, TIMP-2, TIMP-3 was done using RT-QPCR. 

We demonstrated that genistein has the influence on the expression of MMP-1, -3, -14, -13 -15 and TIMP-1 in T47D and HFF-1 cells in the way that implies its capacity to inhibit the process of angiogenesis and metastasis. Though this effect in vitro is not unequivocal, we presume it can be helpful in clinical practice to decrease the risk of angiogenesis and increase survial of patients with breast cancer.


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Presentation: Poster at IX Multidyscyplinarna Konferencja Nauki o Leku, by Małgorzata T. Latocha
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-15 10:58
Revised:   2014-05-02 12:36