Breast cancer is one of the most common cancers among women. Thus, the scientists are still looking for the new efficient therapeutic methods of treatment.

Phytoestrogens are currently in the center of  attention because of their therapeutic and preventive proprieties. Genistein is a naturally occurring plant compound from the group of phytoestrogens which possess many therapeutic activities. Its anticancer effects occur at multiple levels, including cell cycle regulation, induction of programmed cell death and inhibition of angiogenesis and metastasis.

The aim of this study was to examine the impact of genistein on genes involved in MMPs and TIMPs synthesis in breast cancer cells and normal fibroblasts stimulated by medium derived from T-47 D cultures. We used slightly invasive hormone-dependent T-47D (ATCC) breast cancer cells, subtype Luminal A and normal fibroblasts HFF-1 (ATCC) isolated from skin. Quantitation of the genes encoding MMP-1, -2, -3 -9, -13, -14, -15 and tissue inhibitors of metalloproteinase : TIMP-1, TIMP-2, TIMP-3 was done using RT-QPCR. 

We demonstrated that genistein has the influence on the expression of MMP-1, -3, -14, -13 -15 and TIMP-1 in T47D and HFF-1 cells in the way that implies its capacity to inhibit the process of angiogenesis and metastasis. Though this effect in vitro is not unequivocal, we presume it can be helpful in clinical practice to decrease the risk of angiogenesis and increase survial of patients with breast cancer.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/31134 must be provided.

1. The impact of genistein and phytic acid on the viability of nasal polyps cells in an in vitro model
2. Antiproliferative and cytotoxic effect of vitamin D₃ and its derivatives on the selected cells with a higher proliferative potential
3. Molecular effects of newly synthesized derivatives of phenothiazine in C-32 and MDA-MD-231 cancer cells
4. Evaluation of lactase dehydrogenase activity as a marker of carbohydrate metabolism in melanoma cells line Me45 under the influence of cisplatin
5. Effect of doxorubicin on selected oxidative stress markers in adenocarcinoma cell line A549
6. The effect of soybean genistein on the expression of selected genes regulating cell cycle in fibroblasts derived from keloids
7. Evaluation of genistein ability to modulate the protein expression of CTGF and the genes expression of TGF β1, β2 and β3 isoforms in keloid fibroblasts in vitro
8. Polyunsaturated fatty acids potentate cytotoxicity of cisplatin in A549 cells
9. Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on the TNF-α – dependent IL-6 secretion in normal human dermal fibroblasts
10. The influence of inositol hexaphosphate on the expression of genes encoding matrix metalloproteinases 2 and 9 and their tissue inhibitors in human colon cancer cells
11. Chemical characteristics of melanin from the human melanoma malignum
12. Expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 in laryngeal squamous cell carcinoma
13. The influence of doxorubicin on the activity of lactase dehydrogenase of squamous cell carcinoma cells AT478 in vitro
14. The thermochemolysis of melanins isolated from melanoma malignum