There are many reports in the literature on multidirectional activity of vitamin D and its analogs. Recently, the role of vitamin D derivates as potential anti-proliferative agents in addition to the well-known application in the treatment of rickets and osteoporosis has been revealed. Studies on the mechanisms of action of vitamin D and its analogs demonstrated that calcitriol, hormonally active form of vitamin D, has a regulatory effect toward cells’ proliferation, division and differentiation. Numerous researches revealed also the influence of those substances on apoptosis and inhibition of cell proliferation in certain types of malignant tumors.
The aim of the present study was to evaluate the in vitro effect of new derivatives of vitamin D₃ on the divisions and viability of the cells with increased proliferative activity. The effect of calcitriol, tacalcitol and three new derivatives of vitamin D₃ synthesized at the Pharmaceutical Research Institute in Warsaw, coded as PRI-1890, PRI-1906 and PRI-2205 was examined.
All the tests were performed on tumor cell lines (SNB-19, C-32 and SH-4), cultures derived from nasal polyp tissues and cultures of normal skin fibroblasts (HFF-1). Cultures were set and carried out in 75ml Nunc cell culture vessel, then the cells were seeded into 96-well plates and after 24h incubation calcitriol, tacalcitol, RPI-1890, RPI-1906 and RPI-2205 at a concentration of 0.025, 0.25, 2.5 and 25ug/ml were added to the medium. After subsequent 72h the number of cells was determined in cultures using the WST-1 assay (Roche Molecular Biochemicals). We analyzed both the survival and proliferative activity of cells treated with the above derivatives of vitamin D₃. RT-QPCR technique was used to determine the expression of anti-apoptotic Bcl -2 gene and pro-apoptotic BAX gene.

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