Keloids characterize overgrowth of connective tissue in the skin that arises as a consequence of abnormal wound healing. Normal wound healing is regulated by a complex set of interactions within a network of profibrotic and antifibrotic cytokines that regulates new extracellular matrix (ECM)  synthesis and remodeling. These proteins include transforming growth factor-β (TGF-β) and connective tissue growth factor (CTGF). TGF-β stimulates fibroblasts to synthesis and contraction of ECM and acts as a central mediator of profibrotic response. CTGF is induced by TGF-β and is considered as a downstream mediator of TGF-β action in fibroblasts. CTGF plays a crucial role in keloid pathogenesis by promoting prolonged collagen synthesis and deposition and in a consequence sustained fibrotic response.

Genistein (4,5,7-trihydroxyisoflavone) exhibits multidirectional biological action. Genistein shows numerous intracellular targets of actions in live cells such as estrogen  receptors (ERs), tyrosine-specific protein kinases and transcription factors etc. NFΚB, Akt and AP-1 engaged in cytokines expression.

The aim of the study was to investigate the effect of genistein on the expression of CTGF and TGF β1, β2 and β3 isoforms in keloid fibroblast cultured in vitro. Normal dermal fibroblasts were used as a control cells.

Natural soybean genistein in: 370 µM, 185 µM, 92,5 µM, 37µM, 18,5 µM, 3,7 µM, 1,85 µM and 0,185 µM concentrations were used in the study. Real time RT-QPCR was used to estimate transcription level of CTGF and TGF β1, β2 and β3 isoforms in normal and keloid fibroblasts treated with genistein.

Secreted/cell-associated CTGF was evaluated in cell medium by ELISA. Total protein quantification was evaluated by fluorymetric assay in cells lysates (Quant-iT TM Protein Assay Kit).

Results: TGF β1, β2 and β3 genes expression are modulated by genistein in concentration dependent manner. Genistein suppresses the expression of mRNA CTGF and its protein.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/31090 must be provided.

1. The impact of genistein and phytic acid on the viability of nasal polyps cells in an in vitro model
2. Antiproliferative and cytotoxic effect of vitamin D₃ and its derivatives on the selected cells with a higher proliferative potential
3. The transcripional activity of genes encoding MMPs and TIMPs in breast cancer cells treated by genistein and in normal cancer-associated fibroblasts - in vitro studies
4. Molecular effects of newly synthesized derivatives of phenothiazine in C-32 and MDA-MD-231 cancer cells
5. Evaluation of lactase dehydrogenase activity as a marker of carbohydrate metabolism in melanoma cells line Me45 under the influence of cisplatin
6. Effect of doxorubicin on selected oxidative stress markers in adenocarcinoma cell line A549
7. The effect of soybean genistein on the expression of selected genes regulating cell cycle in fibroblasts derived from keloids
8. Polyunsaturated fatty acids potentate cytotoxicity of cisplatin in A549 cells
9. Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on the TNF-α – dependent IL-6 secretion in normal human dermal fibroblasts
10. The influence of inositol hexaphosphate on the expression of genes encoding matrix metalloproteinases 2 and 9 and their tissue inhibitors in human colon cancer cells
11. Chemical characteristics of melanin from the human melanoma malignum
12. Expression of matrix metalloproteinase 2 and matrix metalloproteinase 9 in laryngeal squamous cell carcinoma
13. The thermochemolysis of melanins isolated from melanoma malignum