Search for content and authors

Evaluation of genistein ability to modulate the protein expression of CTGF and the genes expression of TGF β1, β2 and β3 isoforms in keloid fibroblasts in vitro

Magdalena E. Jurzak 1Katarzyna Adamczyk Paweł Antończak Agnieszka Garnacarczyk Dariusz Kuśmierz 2Małgorzata T. Latocha 2

1. Department of Cosmetology Medical University of Silesia (SUM), Kasztanowa 3, Sosnowiec 40-055, Poland
2. Department of Cell Biology, Medical University of Silesia (SUM), Jedności 8, Sosnowiec 41-200, Poland


Keloids characterize overgrowth of connective tissue in the skin that arises as a consequence of abnormal wound healing. Normal wound healing is regulated by a complex set of interactions within a network of profibrotic and antifibrotic cytokines that regulates new extracellular matrix (ECM)  synthesis and remodeling. These proteins include transforming growth factor-β (TGF-β) and connective tissue growth factor (CTGF). TGF-β stimulates fibroblasts to synthesis and contraction of ECM and acts as a central mediator of profibrotic response. CTGF is induced by TGF-β and is considered as a downstream mediator of TGF-β action in fibroblasts. CTGF plays a crucial role in keloid pathogenesis by promoting prolonged collagen synthesis and deposition and in a consequence sustained fibrotic response.

Genistein (4,5,7-trihydroxyisoflavone) exhibits multidirectional biological action. Genistein shows numerous intracellular targets of actions in live cells such as estrogen  receptors (ERs), tyrosine-specific protein kinases and transcription factors etc. NFΚB, Akt and AP-1 engaged in cytokines expression.

The aim of the study was to investigate the effect of genistein on the expression of CTGF and TGF β1, β2 and β3 isoforms in keloid fibroblast cultured in vitro. Normal dermal fibroblasts were used as a control cells.

Natural soybean genistein in: 370 µM, 185 µM, 92,5 µM, 37µM, 18,5 µM, 3,7 µM, 1,85 µM and 0,185 µM concentrations were used in the study. Real time RT-QPCR was used to estimate transcription level of CTGF and TGF β1, β2 and β3 isoforms in normal and keloid fibroblasts treated with genistein.

Secreted/cell-associated CTGF was evaluated in cell medium by ELISA. Total protein quantification was evaluated by fluorymetric assay in cells lysates (Quant-iT TM Protein Assay Kit).

Results: TGF β1, β2 and β3 genes expression are modulated by genistein in concentration dependent manner. Genistein suppresses the expression of mRNA CTGF and its protein.


Legal notice
  • Legal notice:

Related papers

Presentation: Poster at IX Multidyscyplinarna Konferencja Nauki o Leku, by Magdalena E. Jurzak
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-14 12:39
Revised:   2014-05-02 10:53