Search for content and authors

The effect of soybean genistein on the expression of selected genes regulating cell cycle in fibroblasts derived from keloids

Magdalena E. Jurzak ,  Katarzyna Adamczyk ,  Agnieszka Garnacarczyk ,  Paweł Antończak 

Department of Cosmetology Medical University of Silesia (SUM), Kasztanowa 3, Sosnowiec 40-055, Poland


Keloids are bening fibroproliferative tumors considered to be scars that results when wounds heal abnormally. The underlaying pathophysilogical mechanism is unclear. However disturbed cell cycle regulation is observed in keloids. The process plays an important role in maintaining genetic integrity of the cell. P53 can arrest human cells with damaged DNA in G1 phase of cell cycle, that allows DNA repair before S-phase. If the DNA damage is severe and cannot be repaired, apoptotic pathways are activated to eliminate damaged cells. P53 as a transactivator of transcription can induce apoptosis by up-regulation of pro-apoptotic gene expression such as BAX and down-regulation of antiapoptotic genes expression such as BCL-2.

Genistein (4,5,7-trihydroxyisoflavone) exhibits multidirectional biological action. The concentration of genistein is relatively high in soybean. Genistein has been shown as effective antioxidant and chemopreventive agent. Genistein can  bind to estrogen receptors (ERs) and modulate estrogen action due to its structure similarity to human estrogens. Genistein also inhibits transcription factors NFΚB, Akt and AP-1 signaling pathways, that are important for cell proliferation, differentiation, survival and apoptosis.

The aim of the study was to investigate genistein as a potential regulator of  TP53, CDKN1A, BAX and BCL-2  in normal fibroblasts and fibroblasts derived from keloids cultured in vitro.

Natural soybean genistein in: 370 µM, 185 µM, 92,5 µM, 37µM, 18,5 µM, 3,7 µM, 1,85 µM and 0,185 µM concentrations were used in the study. Real time RT-QPCR was used to estimate transcription level of selected genes in normal and keloid fibroblasts treated with genistein.

Results: TP53 and CDKN1A genes expression are modulated by genistein in concentration dependent manner. The agent also modulates BAX/BCL-2  ratio in examined cells.


Legal notice
  • Legal notice:

Related papers

Presentation: Poster at IX Multidyscyplinarna Konferencja Nauki o Leku, by Magdalena E. Jurzak
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-14 11:51
Revised:   2014-05-02 10:54