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The impact of genistein and phytic acid on the viability of nasal polyps cells in an in vitro model

Marcin Frączek 1Dariusz Kuśmierz 2Beata Rostkowska-Nadolska 1Tomasz Kręcicki 1Małgorzata T. Latocha 2

1. Wroclaw Medical University, Dept. of Otolaryngology (WMU), Borowska 213, Wrocław 50-556, Poland
2. Department of Cell Biology, Medical University of Silesia (SUM), Jedności 8, Sosnowiec 41-200, Poland


In developed countries, chronic rhinosinusitis with polyps is one of the several diseases that diminish patients’ quality of life most significantly. The treatment of that often incurable disease is mainly based on the intranasal steroids and surgery in patients who had failed thorough conservative management. The frequent recurrence of inflammatory process triggers a need to repeat the operation. It appears that the introduction of new treatment substance that inhibits fibroblasts’ proliferation, decreases pro-inflammatory stimulation and suppress inflammation process in the tissue (i.e. eosinophils and mast cells reduction, decreased level of inflammatory cytokines, such as IL-5, IL-6 and IL-8) would be a valuable therapeutic option. Currently, steroids are the most commonly used for that purpose what is associated with the occurrence of problematic side effects forcing many patients to discontinue therapy. Increasing interest in this field raise natural substances such as flavonoids. In addition to its antioxidant properties, they often have a capacity to influence the course of the cell cycle, inhibit cell division, induce apoptosis and change various metabolic pathways.

The aim of the present study was to evaluate the in vitro effects of genistein and phytic acid (IP6) on the viability and proliferative activity of cells derived from nasal polyps. Material for the study was obtained from patients with chronic rhinosinusitis with polyps surgically treated in the Department of Otolaryngology, Medical University in Wroclaw. The patients included into the study were free of any medication at least 4 weeks prior to surgery. The study used cells from IV-VI passage.

Cells viability and proliferation activity were analyzed in cultures in the presence of various concentrations of genistein (0.1-500 uM) and IP6 (0.1-20 mM). WST-1 test (Roche Molecular Biochemicals) and the measurements of LDH concentration in the medium were used for that purpose. An immunocytochemical assay was performed to demonstrate the possible presence of apoptotic or necrotic cells.

For both of the tested substances - stronger for genistein than IP6, concentration-dependent effect on the parameters analyzed was demonstrated.

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Submitted: 2014-03-15 09:00
Revised:   2014-04-28 18:57