In developed countries, chronic rhinosinusitis with polyps is one of the several diseases that diminish patients’ quality of life most significantly. The treatment of that often incurable disease is mainly based on the intranasal steroids and surgery in patients who had failed thorough conservative management. The frequent recurrence of inflammatory process triggers a need to repeat the operation. It appears that the introduction of new treatment substance that inhibits fibroblasts’ proliferation, decreases pro-inflammatory stimulation and suppress inflammation process in the tissue (i.e. eosinophils and mast cells reduction, decreased level of inflammatory cytokines, such as IL-5, IL-6 and IL-8) would be a valuable therapeutic option. Currently, steroids are the most commonly used for that purpose what is associated with the occurrence of problematic side effects forcing many patients to discontinue therapy. Increasing interest in this field raise natural substances such as flavonoids. In addition to its antioxidant properties, they often have a capacity to influence the course of the cell cycle, inhibit cell division, induce apoptosis and change various metabolic pathways.

The aim of the present study was to evaluate the in vitro effects of genistein and phytic acid (IP6) on the viability and proliferative activity of cells derived from nasal polyps. Material for the study was obtained from patients with chronic rhinosinusitis with polyps surgically treated in the Department of Otolaryngology, Medical University in Wroclaw. The patients included into the study were free of any medication at least 4 weeks prior to surgery. The study used cells from IV-VI passage.

Cells viability and proliferation activity were analyzed in cultures in the presence of various concentrations of genistein (0.1-500 uM) and IP6 (0.1-20 mM). WST-1 test (Roche Molecular Biochemicals) and the measurements of LDH concentration in the medium were used for that purpose. An immunocytochemical assay was performed to demonstrate the possible presence of apoptotic or necrotic cells.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/31128 must be provided.

1. Antiproliferative and cytotoxic effect of vitamin D₃ and its derivatives on the selected cells with a higher proliferative potential
2. The transcripional activity of genes encoding MMPs and TIMPs in breast cancer cells treated by genistein and in normal cancer-associated fibroblasts - in vitro studies
3. Molecular effects of newly synthesized derivatives of phenothiazine in C-32 and MDA-MD-231 cancer cells
4. Evaluation of lactase dehydrogenase activity as a marker of carbohydrate metabolism in melanoma cells line Me45 under the influence of cisplatin
5. Effect of doxorubicin on selected oxidative stress markers in adenocarcinoma cell line A549
6. Evaluation of genistein ability to modulate the protein expression of CTGF and the genes expression of TGF β1, β2 and β3 isoforms in keloid fibroblasts in vitro
7. Polyunsaturated fatty acids potentate cytotoxicity of cisplatin in A549 cells
8. Chemical characteristics of melanin from the human melanoma malignum
9. The thermochemolysis of melanins isolated from melanoma malignum