Search for content and authors

Morphological picture of kidney and the activity of selected enzymes after administration of vitamin E and methionine in rats exposed to sodium fluoride.

Beata Bielec 1Barbara Stawiarska-Pięta 1Aleksandra Iskra 1Agata Kabała-Dzik 1Robert Kubina 1Jolanta E. Zalejska-Fiolka 2Natalia Grzegorzak 1Ewa Birkner 2

1. Department of Pathology, Faculty of Pharmacy, Silesian Medical University in Katowice, Ostrogórska 30, Sosnowiec 41-200, Poland
2. Department of General Biochemistry, Faculty of Medicine in Zabrze, Silesian Medical University, Jordana 19, Zabrze 41-808, Poland


In doses exceeding the dose limit fluoride ions have multidirectional toxic effect on living organism. They induce pathomorphological changes in the parenchymal organs and affect the metabolic pathways of, lipids, proteins and carbohydrates. DNA damage of the cells is also observed. In recent studies have been proven that important role in causing the observed pathological changes plays disorders oxidoreduction processes.

The experiment was conducted in 18 male rats of Wistar strain, which average body mass before the experiment was 393,39 ± 47,54 g. The rats were divided into three groups with 6 animals in each group. Rats from control group were given distilled water The animals in both experimental group I and II received 10 mg NaF/kg of body mass/24 h in water solution. In addition, second experimental group of rats was administered methionine (2 mg/rat/24 h) and vitamin E (3 mg/rat/24 h) with NaF. The experiment lasted 35 days. After this time the animals were weighed, then put to sleep by means of an intraperitoneal administration of hexobarbital. Upon section kidneys of rats were removed and were fixed in formalin. Preparations were made by means of the paraffin method and were stained with hematoxylin and eosin. In the supernatants obtained after homogenization in saline and centrifugation the activity of enzymes of carbohydrate metabolism: fructose-1,6-bisphosphate aldolase (ALD), malate dehydrogenase (MDH), lactate dehydrogenase (LDH), sorbitol dehydrogenase (SDH) and glutathione-S-transferase (GST), gluthatione reductase (GR), gluthatione peroxydase (GPx) was measured.

The study showed an adverse effect of fluoride on kidneys histopathology. The edema, disseminated necrosis of renal tubular epithelial cells and hyperaemia of nutrient capillary of the renal tubules were noticed. In addition, there were narrowing Bowman space and hyperaemia of the glomerulal capillary. Furthermore small infiltration of mononuclear cells in the stroma was observed too. Exposure to NaF adversely affected also to the activity of enzymes of carbohydrate metabolism (decreased activity). A statistically significant decrease in activity of MDH was observed which indicates alterations of energy processes of the cell. Interference in the Krebs cycle’s has been shown too. Whereas the activity enzymes associated with gluthatione was slightly reduced in rats intoxicated with NaF.

The combined administration of antioxidants showed beneficial effects upon activity of enzymes in the kidney of rats exposed to sodium fluoride and inhibited the pathomorphological alterations which developed in kidneys following sodium fluoride intoxication.


Legal notice
  • Legal notice:

Related papers

Presentation: Poster at XXXth Conference of the International Society for Fluoride Research, by Beata Bielec
See On-line Journal of XXXth Conference of the International Society for Fluoride Research

Submitted: 2012-06-27 21:51
Revised:   2012-07-23 11:27