In doses exceeding the dose limit fluoride ions have multidirectional toxic effect on living organism. They induce pathomorphological changes in the parenchymal organs and affect the metabolic pathways of, lipids, proteins and carbohydrates. DNA damage of the cells is also observed. In recent studies have been proven that important role in causing the observed pathological changes plays disorders oxidoreduction processes.

The experiment was conducted in 18 male rats of Wistar strain, which average body mass before the experiment was 393,39 ± 47,54 g. The rats were divided into three groups with 6 animals in each group. Rats from control group were given distilled water The animals in both experimental group I and II received 10 mg NaF/kg of body mass/24 h in water solution. In addition, second experimental group of rats was administered methionine (2 mg/rat/24 h) and vitamin E (3 mg/rat/24 h) with NaF. The experiment lasted 35 days. After this time the animals were weighed, then put to sleep by means of an intraperitoneal administration of hexobarbital. Upon section kidneys of rats were removed and were fixed in formalin. Preparations were made by means of the paraffin method and were stained with hematoxylin and eosin. In the supernatants obtained after homogenization in saline and centrifugation the activity of enzymes of carbohydrate metabolism: fructose-1,6-bisphosphate aldolase (ALD), malate dehydrogenase (MDH), lactate dehydrogenase (LDH), sorbitol dehydrogenase (SDH) and glutathione-S-transferase (GST), gluthatione reductase (GR), gluthatione peroxydase (GPx) was measured.

The study showed an adverse effect of fluoride on kidneys histopathology. The edema, disseminated necrosis of renal tubular epithelial cells and hyperaemia of nutrient capillary of the renal tubules were noticed. In addition, there were narrowing Bowman space and hyperaemia of the glomerulal capillary. Furthermore small infiltration of mononuclear cells in the stroma was observed too. Exposure to NaF adversely affected also to the activity of enzymes of carbohydrate metabolism (decreased activity). A statistically significant decrease in activity of MDH was observed which indicates alterations of energy processes of the cell. Interference in the Krebs cycle’s has been shown too. Whereas the activity enzymes associated with gluthatione was slightly reduced in rats intoxicated with NaF.

The combined administration of antioxidants showed beneficial effects upon activity of enzymes in the kidney of rats exposed to sodium fluoride and inhibited the pathomorphological alterations which developed in kidneys following sodium fluoride intoxication.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28268 must be provided.

1. Evaluation of lactase dehydrogenase activity as a marker of carbohydrate metabolism in melanoma cells line Me45 under the influence of cisplatin
2. Effect of doxorubicin on selected oxidative stress markers in adenocarcinoma cell line A549
3. The influence of lipoic acid and vitamins A and E on the selected biochemical parameters in the kidneys of rats intoxicated with sodium fluoride
4. Beneficial effects of lipoic acid and some vitamins on fluoride toxicity in liver of rats
5. Vascular Cell Adhesion Molecule-1 as a risk factor of atherosclerosis in diabetes mellitus patients.
6. The influence of diet supplementation with methionine and vitamin E on the morphological picture of rats organs intoxicated with sodium fluoride
7. The influence of non-oxidized and oxidized rapeseed oil with alpha-lipoic acid addition on lipids profile and paraoxonase-1 activity in rats
8. The influence of doxorubicin on the activity of lactase dehydrogenase of squamous cell carcinoma cells AT478 in vitro
9. The influence of methionine and combination of methionine with vitamin E upon the activity of antioxidant enzymes, lipid and cholesterol oxidation in the blood of rabbits with experimental hypercholesterolemia.
10. Plasma and synovial fluid lipid peroxidation and nonenzymatic antioxidants in patients with primary osteoarthritis of the knee.
11. Lipid peroxidation and non-enzymatic status in lead exposed workers.