As in the case of many degenerative diseases, increased production of reactive oxygen species (ROS) and lipid peroxidation fluoride has been considered to play an important role in pathogenesis of on chronic fluoride toxicity [1,2]. Recent studies have shown that the modifications of anitioxidant defense system (enzymatic and nonenzymatic) in fluorosis were observed [2, 3, 4]. Vitamines A, E and C and as well as methionine and co-enzyme Q prevent the organism from the activity of free oxidative radicals. Usuelly vitamin E is administrated along with methionine or vitamin C.

The aim of the study was the influence of methionine and vitamin E on the development of pathomorphological changes in the lungs and pancreases of rats intoxicated with fluoride sodium. The study was performed on Wistar FL 18 rats (adult males). The animals were divided into 3 groups. Rats in control group were kept on distilled drinking water. Animals in study group I and II were supplemented with  10 mg sodium fluoride/ kg b.m./24h. Additionally, animals in study group II supplemented diet providing 3 mg of vitamin E/rat/24h and 2 mg methionine/rat/24h.

The experiment lasted for 35 days. Then the rats were intraperitioneally anesthetized, using thiopental at a dose of 30 mg/rat. Blood was collected for biochemical analyses and during the post-mortem examination were collected: lungs and pancreases for histopathological tests. The pathomorphological changes in the organs were assessed on the basis of paraffin preparations, stained with hematoxilin and eosin (H-E). The aldolase (ALD) and malate dehydrogenase (MDH) activities in the pancreas were determinated with colorimetric methods.

Pathomorphological examinations of lungs revealed, in the case of NaF administration, that examined rats developed hyperemia, erythrorrhagia, inflammatory infiltrations, emphysematous blebs. There were focal vacuolar degeneration cells and inflammatory infiltrations noticed only in pancreases.

It was proved that the administered methionine and vitamin E inhibited the hyperemia and regressive changes in lungs and pancreases of rats intoxicated with sodium fluoride. No statistically significant change in the ALD activity in the pancreas was observed in the study groups. There was a statistical increase - when compared to the control group - in the MDH activity in study groups.

References:

1 Machoy Z (1987) Folia Med. Cracoviensa. 28(1-2): 61-81.

2 Chlubek D et al (2001) Bromat. Chem. Toxikol. XXXIV(3):263-266.

3 Shivarajashankara Y.M et al ( 2001) Fluoride. 34(2): 108-113.

4 Xiu-an Zhanet et al (2005)Fluoride. 38(2): 157-161

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