The study was undertaken to determine the effect of methionine and combination of methionine with vitamin E on induced hypercholesterolemia in rabbits, measured as antioxidant enzymes (superoxide dismutase –SOD, glutathione peroxidase – GPX and catalase – CAT) as well as lipid (as malondialdehyde – MDA) and cholesterol (as 7-ketocholesterol) oxidation products in blood. The animals were received diet rich in cholesterol 0.5 g% with the addition of methionine in food in the dose of 70 mg/kg/24h or in combination with DL-a-tocopherol in food (10mg/kg/24h) for 3 months.

It was found that the addition of methionine to fodder enriched with cholesterol initially had a beneficial influence upon the concentration of MDA, and upon the activity of the SOD but the addition of methionine together with vitamin E leads to substantial reduction of the concentration of MDA and 7-ketochoelstrol in blood serum, with simultaneous increase of the SOD activity in serum and erythrocytes and well as CAT and GPX in the erythrocytes.

One can conclude that a joint administration of methionine and vitamin E effectively protects the cell from lipid peroxidation. It seems that beneficial effect of combination these compounds suggests a role of methionine in the regeneration of tocopherol radical and influence to the antioxidant system.

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