Flax is one of the oldest crop plants. Various parts of the plant have been used to make fabric, dye or paper. Flax is also known for its beneficial effect on health. The linseed supplies about 8% mucilages, from 30 to 40% of oil, 25% of proteins, glucosides, sterols, enzymes, mineral salts and other compounds.

Rich composition of the bioactive compounds included in flax, especially proteins like cyclolinopeptides (cyclolinopeptide A, naturally existing immunomodulatory peptide) induced us to interest in this topic. The cyclolinopeptides are included not only in the linseed, but also in the scrap material, roots, oilcakes and chaff.

The purpose of the work was isolated fraction of cyclolinopeptides from oilcakes and chaffs. The individual cyclolinopeptide were separated with chromatographic method and identified by spectroscopic methods.

To solvent extraction method various organic solvents (hexane, cyclohexane and toluene) have been used for examinations. The process of the extraction was carried out periodically with solvents in the room temperature and in their boiling point.

The percentage content of cyclolinopeptide A in individual fractions was established by the HPLC method.

The method of distinguishing the cyclolinopeptide CLA from linseed cakes was drawn up. The first step was the solvent extraction in the room temperature, and then the threefold extraction in the boiling point. The preliminary extraction was applied to removed oil remains from oilcakes.

Cyclolinopeptide A was obtained with high productivity about 0.02 % (in converting into the plant material) the content of cyclolinopeptide A in plant material amount 0.66 %. Moreover, the LC-MS/MS method confirmed that the extract from linseed cakes contains cyclolinopeptide A and comparable amounts of cyclolinopeptide E.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/23043 must be provided.

1. Synthesis of trans-4-cyclohexyl-(S)-proline - an intermediate in Fosinopril process
2. Application of Corona detector for analysis of 4-cyclohexyl-(S)-proline – key intermediate in Fosinopril synthesis
3. An efficient preparation of 3,5-bis(2-cyanoisopropyl) toluene-key intermediate in Anastrozole synthesis
4. Anti-oxidativeproperties of prenylflavonoids isolated from hops (Humulus lupulus L.)
5. Improvement of the Phase-Transfer Catalysis (PTC) method for synthesis of L-proline derivatives
6. Separation of enantiomers of Z and E 3-phenylglycidic acid t-butyl ester
7. Isolation and purification of (2R,3S)-3-phenylisoserine hydrochloride
8. Phase Transfer Catalysis in pharmaceutical industry – where are we?
9. SYNTHESIS AND HYPOLIPIDEMIC ACTIVITY OF ASARONE ANALOGS IN MALE AND FEMALE RATS
10. COMPARISON OF THE STABILITY OF 5-AMINOLEVULINIC ACID AND HIS ESTERS
11. THE INFLUENCE OF TEMPERATURE AND SOLVENT ON DARZENS CONDENSATION STEREOSELECTIVITY
12. APPLICATION OF HPLC FOR ANALYTICAL CONTROL OF LARGE SCALE SYNTHESIS OF (2R, 3S)-3-PHENYLISOSERINE HYDROCHLORIDE
13. LARGE SCALE LABORATORY SYNTHESIS OF 1-(4-CHLOROPHENYL)-1-CYANOCYCLO- BUTANE - A SIBUTRAMINE PRECURSOR