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Molecularly imprinted polymer based electrochemical sensors for flow injection determination of adrenaline

Tan-Phat Huynh 1Chandra Bikram KC 3Janusz W. Sobczak 1Francis D. Souza 3Wlodzimierz Kutner 1,2

1. Polish Academy of Sciences, Institute of Physical Chemistry, Kasprzaka 44/52, Warszawa 01-224, Poland
2. Cardinal Stefan Wyszynski University in Warsaw, School of Sciences, Faculty of Mathematics and Natural Sciences, Dewajtis 5, Warsaw 01-815, Poland
3. University of North Texas Denton, Department of Chemistry, 1155 Union Circle 305070 TX, Denton 76203, United States


The electrochemical sensors were fabricated for selective and sensitive determination of adrenaline.  In these sensors, a thin film of the adrenaline-templated molecularly imprinted polymer (MIP) was used for adrenaline recognition and differential pulse voltammetry (DPV) and piezoelectric microgravimetry (PM) for analytical signal transduction under flow-injection analysis conditions. First, molecular structure of a complex (Scheme 1) of protonated adrenaline (HAd) with the benzo-(18-crown-6) (Cr6BTP) and benzoic acid (COOHBTP) derivatives of bis(2,2’-bithienyl)methane, used as electroactive functional monomers, was optimized with the B3LYP/6-31g(d) density functional theory method by the Gaussian 03 software.  Then, the MIP film was deposited on a Pt disk electrode and 10 MHz quartz crystal resonator (QCR) by potentiodynamic electropolymerization from a solution of HAd, Cr6BTP, CHOBTP, and the cross-linking tris(2,2’-bithienyl)methane monomer at the mole ratio of 1:1:1:2.  Next, the adrenaline template was extracted with 0.01 M NaOH from the MIP film; completeness of the extraction was confirmed by XPS.  For adrenaline determination, the decrease of the DPV peak current for the 0.1 M K4[Fe(CN)6] redox probe and the change of the resonance frequency of the 10 MHz QCR, proportional to the adrenaline concentration, was measured.


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Related papers

Presentation: Short communication at SMCBS'2011 International Workshop, by Tan-Phat Huynh
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-10-19 21:20
Revised:   2011-10-19 21:20