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Growth of human fibroblasts in the presence of 6-hydroxyhexanoic acid

Arkadiusz Orchel 1Monika A. Musiał-Kulik 1Katarzyna Jelonek 2Janusz Kasperczyk 1,2Zofia Dzierżewicz 1

1. Department of Biopharmacy, Medical University of Silesia, Narcyzów 1, Sosnowiec 41-200, Poland
2. Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze 41-819, Poland

Biodegradable polymers containing caproyl units belong to the most popular materials used widely in medicine, pharmacy (as drug delivery systems) and tissue engineering. Recently co- and terpolymers obtained from poly-ε-caprolactone have been found to possess a shape memory effect. Shape memory devices are suitable for a wide range of medical applications such as biodegradable stents or surgical clamps. The degradation product of poly-ε-caprolactone hydrolysis is 6-hydroxyhexanoic acid. It is thought to be biocompatible but there are not any experimental data that demonstrate its impact on human cells in vitro.

The aim of our study was todetermine the impact of different concentrations of 6-hydroxyhexanoic acid on human fibroblasts (HGF-1 cell line). Changes in cell morphology and growth rate were analyzed as well as the concentration of tested compound in cell culture medium was assessed.

6-hydroxyhexanoic acid was used at a concentrations range of 0,1–20 mM. Cell proliferation was measured using sulforhodamine B, a dye binding to cellular proteins. Concentration of the studied compound in culture medium was assessed using HPLC technique.

Fibroblasts growing in the presence of6-hydroxyhexanoic acid at all concentrations displayed normal morphology, remained spread on the substratum and any cell detachment was not observed. Some inhibition of cell growth was seen exclusively at the highest concentration of the tested substance.

Generally, results of our study evidence the biocompatibility of the degradation product of poly-ε-caprolactone.

Acknowledgement: This study has been financially supported by MEMSTENT (Grant No: UDA-POIG.01.03.01-00-123/08-01).


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Presentation: Poster at VII Multidyscyplinarna Konferencja Nauki o Leku, by Arkadiusz Orchel
See On-line Journal of VII Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2010-03-15 13:24
Revised:   2010-03-15 13:25