Aldehyde dehydrogenase isozyme (ALDH3A1) is a dimeric enzyme oxidizing mainly long- and medium-chain aliphatic, as well as aromatic, aldehydes, but exhibiting very low affinity toward acetaldehyde. ALDH3A1 isozyme, is responsible for detoxication of many drugs, including an alkylating agent cyclophosphamide (CP) widely utilized in cancer chemotherapy, usually in combination with other drugs [1, 2, 3].

It has been recently shown that ALDH3A1 is the only aldehyde dehydrogenase isozyme present in human saliva [4]. It is also well documented that ALDH3A1 can be induced several-hundred-fold in some neoplastic states of the different cancers e.g. liver, breast, and colon [5].

We have applied the fluorimetric method to study aldehyde dehydrogenase isozyme (ALDH3A1) activity in saliva of cancerous patients. A highly fluorogenic aldehyde, 6-methoxy-2-naphthaldedyde (MONAL-62) was used as an indicator of the aldehyde dehydrogenase isozyme (ALDH3A1) in human saliva [6, 7].

Cancerous patients' saliva was collected and examined over a prolonged time, both prior to and after tumor removal. In most cases studied thus far, ALDH3A1 activity was initially elevated, but in consecutive assays after surgery significantly decreased. This phenomenon could indicate that this isozyme was induced in malignant tissue, resulting in increasing it's total activity in saliva.

1. Lindahl R. Crit. Rev. Biochem. Mol. Biol., 1992, 27, 287-335.

2. Wang S-L. Wu Ch.-W. Cheng T-C. and Yin S-J. Biochem. Int., 1990, 22, 199-204.

3. Sladek N.E. J. Biochem. Mol. Toxicol., 2003, 17, 7-23.

4. Dyck L.E. Alcohol Clin. Exper. Res., 1995, 19, 420-426.

5. Sreerama L. Hedge M.W. Sladek N.E., Clin. Can. Res., 1995, 1, 1153-1163

6. Wierzchowski J. Wroczyński P. Laszuk K. Interewicz E. Anal. Biochem., 1997, 245, 69-78.

7. Wroczyński P., Wierzchowski J., Analyst, 2000, 125, 511-516.

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