Salivary aldehyde dehydrogenase (ALDH3A1) is the only dehydrogenase detected in saliva. The enzyme oxidizes aromatic and long chain aliphatic aldehydes preserving oral cavity from aldehydes derived from food, air pollution. Therefore, its activity may also be an important factor in prevention of chemical carcinogenesis. ALDH3A1 due to sulphydryl groups in its active site is exposed to oxidation even by oxygen from air. The average inactivation of the enzyme in healthy group is about 80% in morning saliva and depends on coffee, drug consumption and age. The inactivation degree is very variable during a day as well as both intra- and interpersonally.  Causes of that inactivation have been still examining.
The aim of the present study was to determine influence of salivary antioxidant status (salivary peroxidase (SPO), superoxide dismutase (SOD) activity, uric acid (UA) concentration and total antioxidant capacity (ORAC)) on ALDH3A1 inactivation degree in smoking (SG, N=38) and no-smoking group (NSG, N=42). Fasting saliva samples were collected in the morning or during a day.   
ALDH3A1 inactivation, ORAC value and SPO activity were determined fluorometrically. SOD activity and UA concentration was measured photometrically.
Average inactivation degree in SG was higher both in the morning (p=0.0054) and during a day (p=0.00189) in comparison to NSG. Inactivation degree in morning saliva correlated with SPO (r=-0.28, p=0.0317), which level was slightly higher in SG. In smokers the correlation was more prominent (r=-0.62, p=0.00121), whereas in NSG was close to zero and non significant. In saliva collected during a day inactivation degree correlated with UA (r=-0.30, p=0.02820) and SOD (r=-0.35, p=0.00598). Correlation with UA in no smokers was more prominent (r=-0.46, p=0.00847) whereas in smoking group was not visible. Moreover, SOD activity in SG was higher than in NSG. No correlations of ALDH inactivation and ORAC value were observed.
To conclude, ALDH3A1 inactivation degree depends on smoking and antioxidant status. In SG depends stronger to antioxidant enzymes than in NSG. Since smoking results in activation of e.g. Nrf2/ARE pathway the inactivation degree in that group is lower than NSG.   

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