Synthesis of multiwalled carbon nanotube-polymer nanohybrids and their internalization to biological systems

Anna Baranowska-Korczyc 1Barbara M. Maciejewska 1,2Małgorzata Jasiurkowska-Delaporte 1Alicja Warowicka 1Grażyna Bartkowiak 1,3Stefan Jurga 1,2

1. Adam Mickiewicz University, NanoBioMedical Centre (CNBM), Umultowska 85, Poznań 61-614, Poland
2. Adam Mickiewicz University, Department of Physics, Umultowska 85, Poznań 61-614, Poland
3. Faculty of Chemistry, Adam Mickiewicz University, Poznań 61-614, Poland

Abstract

Multiwalled carbon nanotubes (MWCNTs) have many potential applications in both, medicine and biology. They can be used either as tissue scaffolds and biomarkers for cellular imaging or drug delivery systems and a basis for biosensors. The MWCNT polymer wraping helps to integrate them into several substance classes or biological systems and make them perfectly dispersed in organic solvents. The CNTs wrapped in polymers are used for the integration into various substance classes or biological systems and enable their dispersibility in organic solvents.

In the present studies, we have focused on the functionalization protocol of multi-walled carbon nanotubes (MWCNTs) by polyethylene glycol (PEG) [1] with three selected molecular weights: 400, 2 000 and 100 000. All three polymers were covalently attached to the surface of previously oxidized carbon nanotubes [2] and the water-soluble PEG-MWCNT hybrids were successfully formed. The nanostructures were characterized by means of Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and Raman spectroscopy. MWCNTs and PEG-MWCNT complexes were internalized into HeLa cells. The cells were analyzed by confocal microscopy and In-cell Analyzer after selected organelles staining. The investigation of PEG-ylated nanostructures treated cells has revealed the higher viability and unchanged morphology in comparison with non functionalized CNT samples.


References:
[1] B. Zhao, H. Hu, A. Yu, D. Peresa, R. C. Haddon, J. Am. Chem. Soc. 127, 8197-8203, 2005.
[2] B. M. Maciejewska, M. Jasiurkowska-Delaporte, A. I. Vasylenko, K. K. Kozioł, S. Jurga, RSC Adv. 4, 28826-28831, 2014.

Acknowledgements
The research was supported by Nation Centre for Research and Development (PBS1/A9/13/2012), the European Social Fund (POKL.04.03.00-00-015/12) and National Science Centre (UMO-2013/11/D/ST5/02900).

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Presentation: Poster at Nano PL 2014, Symposium B, by Anna Baranowska-Korczyc
See On-line Journal of Nano PL 2014

Submitted: 2014-09-17 15:31
Revised:   2014-09-17 15:31
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