Carbon nanostructures include wild range of materials, i.e. carbon nanotubes and nanofibres. There are three forms of nanofibres, classified by the angle of the graphene platelets or layers  with respect to the filament axis: stacked, herringbone and nanotubes. Due to carbon nanostructures properties (i.e. mechanical, electrical, kinetic, optical, thermal) they are commonly used in electronics, mechanics, energy storage, sensors, filling materials in nanocomposites and biomedicine. There are different reports concerning their cytotoxicity profile in biological systems. Especially in the case of herringbone structures, there is poor knowledge of biocompatibility traits. There is deep need to develop bioaspect in this field.

The goal of the study was to determine potential cytotoxic effect of herringbone nanostructures in in vitro study dispersed in two surfactants. 

The investigation were revealed on cancer (HeLa) and normal (HEK293) cell lines. Herringbone nanoparticles in diameter 12 nm, were examined in six different concentrations (5,10,15,25,40,60 µg/ml) as well as in three different time intervals (24, 48 and 72 h). Methodology involved viability test (Trypan blue staining) and WST-1 proliferation assay. All procedures were conducted with two groups of herringbone nanostructures: dispersed in surfactant Nanosperse AQ (NaAQ) (NanoLab Inc) and dispersed in surfactant Pluronic P-123 (Sigma).

The investigations show, that there is no significant cytotoxic effect in analyzed range of nanoparticles’ concentrations in both biological schemes (HEK293 and HeLa cells lines) as well as both surfactants. These results lead to the conclusion, that herringbone carbon nanostructures represent biocompatibility profile with different surfactants, which enable their use in biomedicine. 

Acknowledgment: We would like to acknowledge financial support of the National Centre for Research and Development, Applied Research Programme, The National Centre of Research and Development No PBSll/9ll3l20l2 „Nanomaterials and their potential biomedical applications”

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