Charged aerosol detection (CAD) has been widely employed as an alternative detection system in HPLC. This detection approach was also applied in new UHPLC methods for the determination of protoescigenin (olean-12-ene-3β,16α,21β,22α,24,28-hexaol) assay. Protoescigenin, the main aglycone of escin – a saponin complex from horse chestnut seeds (Aesculus hippocastanum L.) was obtained in scalable, validated manufacturing processes (large laboratory and pilot technical scale) designed and developed in Pharmaceutical Research Institute [1].

In the present work the influence of individual parameters on CAD response and sensitivity was studied. The detection was performed using both CAD and PDA (diode array detector, 200 nm) simultaneously and the results were compared with reference to linearity, accuracy, precision and limit of detection (LOD).

Acknowledgement:
Research Project has been supported from Innovative Economy Operational Programme 2007-2013 funds, under project POIG.0101.02-14-072/09-00 Research on innovative endothelium medicine among novel escin analogues (www.ifarm.eu/poig/escyna/).

References:

[1] M. Gruza, K. Jatczak, B. Zagrodzki, M. Łaszcz, P. Cmoch, K. Woźniak, M. Malińska, T. Giller, K. Koziak, O. Zegrocka-Stendel, G. Grynkiewicz; Preparation, purification and regioselective functionalization of protoescigenin – the main aglycone of escin complex: Molecules 2013, 18(4), 4389-4402; doi:10.3390/molecules18044389

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