Plants are constantly exposed to a large array of pathogenic organisms and the survival in these conditions demands quick defense responses which include the synthesis of defense peptides and proteins with antimicrobial properties. Among these compounds several low molecular weight proteins or peptides with antibacterial or antifungal activity have been isolated in recent years from various plants and are believed to be involved in a defense mechanism by inhibiting microorganisms growth through diverse molecular modes.

The present study has been focused to isolate small proteins/peptides for antimicrobial activity from the plants of Aesculus hippocastanum and Sylibum marianum. The hydrolysates of seed proteins were prepared by using neutral proteases and antimicrobial active fractions were isolated. Low molecular weight peptides generated by cleavage of seed proteins from studied plants showed broad-spectrum antimicrobial activity, inhibiting the growth of bacteria such as Staphylococcus aureus, Pseudomonas aeuroginosa, Escherichia coli and fungi such as Candida albicans.

These antimicrobial proteins in the present study may have commercial value as a natural preservative agent for use in foods and cosmetics and may be used for agricultural or pharmaceutical applications.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/6562 must be provided.

1. Sex differences in the opioid component of swim stress-induced analgesia in mouse lines selected for high or low sensitivity to stress
2. Efficacy of Viburnum opulus fruit juice and its components against herpesvirus
3. New analgesics with adjuvant anticancer properties.
4. Keratin Associeted Proteins as a New Type of Bandage for Wound Healing
5. Development of new multitarget GPCRs ligands (opioid agonist- tachykinin antagonist).
6. Anticancer properties of hair proteins digestive peptides
7. Effects D-tryptophan substitutions on the activity and toxicity of antimicrobial dendrimers
8. Novel amphiphilic dendrimers as antibacterial and antitumoral agents.
9. CLA derivatives as functional food compounds
10. Keratin associated proteins (KAPs) as a new biomaterials for applications in medicine and cosmetology
11. Anti-oxidativeproperties of prenylflavonoids isolated from hops (Humulus lupulus L.)
12. Natural compositions with antioxidative properties
13. Application of fermentation in protein hydrolysates utilisation 
14. Comparable antinociceptive effect of biphalin in mice selected for high and low swim stress-induced analgesia after blood-brain barrier disruption caused by hyperosmolar mannitol administration
15. Application of preparative HPLC for protected peptide purification
16. New tachykinin-opioid chimeric analogues as potential new analgesics
17. Spinal cord peptide epitopes ameliorate immunological reaction in  experimental allergic encephalomyelitis /eae/
18. Dendrimeric peptides with  affinity to opioid receptors - complexation properties
19. Development of dendrimeric somatostatin analogues
20. H-Dmt-D-Lys-Phe-Phe-OH, a tetrapeptide metabolite of the opioid-neurotensin hybrid peptide PK20, expresses high antinociception
21. THERMOSENSITIVE TRI-BLOCK-CO-POLY- MERS POLY- (HPMAm-MONO/DI LACTATE)- PEG-POLY(HPMAm-MONO/DI LACTATE) FOR THE CONTROLLED RELEASE OF OPIOID PEPTIDES
22. HYDROLISATE OF PIG SPINAL CORD AS A NUTRACEUTIC IN MULTIPLE SCLEROSIS.
23. KERATINE PREPARATIONS FOR WOUND HEALING
24. DEVELOPMENT OF NEW TACHYKININ-OPIOID CHIMERIC ANALOGUES AS POTENTIAL NEW ANALGESIC
25. OPIOID PEPTIDE ANALOGUE AA2016 IN MOUSE MODEL OF INDIVIDUAL PAIN SENSITIVITY
26. A SIMPLE PREPARATIVE HPLC ISOLATION OF CONJUGATED LINOLEIC ACIDS (CLAs).