Solid phase methods are the most popular for synthesis of bioactive peptides. In such methods, the peptide is released from solid polymeric resin in its final form. In this case, purification of the product is performed by general HPLC methodology applied for peptides, e.g. acetonitrile/TFA/water gradient. However, particular peptides and/or large preparative scale of syntheses of pepitdes may require purification of the protected peptides. It is known, that most of the protective groups are not stable in standard HPLC conditions.

Recently, we developed variants of classical solution method for economical large scale synthesis of peptides. The intermediate compounds are Boc-protected peptides. Unfortunately, classical physico-chemical methods of purification (extraction, crystalisations, etc) were not very effective in this case. To purify such peptides in large scale we developed HPLC purification conditions that are very economical and safe for the protected-peptides. This rationalizes application of HPLC in technological scale of peptide production even at intermediate stages.

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