When dificulties in receiving crystal structures appear, homology modeling aproach is the best way to obtain three dimensional structure of a protein. Procedure is realtively simple, but results depend on degree of similarity between a target and a template sequence. Since 2000 there was one available crystal structure of transmembrane domain of GPCR protein: the bovine rhodopsin. We, and others have previously shown that regardless of relatively low sequence identity, it was possible to obtain useful rhodopsin based models of serotonin receptors, such as 5-HT_1A and 5HT₇. In October 2007, a high resolution structure of another GPCR, beta-2 adrenergic receptor, was solved. Very close evolutionary relationships between 5-HT receptors and beta adrenoreceptors give us possibility to improve comparative models of serotonin receptors and to verify our previous results. New homology models will be used in a process of drug design and virtual screening.

Acknowledgement
This study was partly supported by the Network "Synthesis, structure and therapeutic properties of compounds and organic substances" coordinated by the Institute of Organic Chemistry Polish Academy of Sciences.

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