Fenoterol is a sympathomimetic directly stimulating bronchial β₂-adrenoreceptors [1] used in bronchial obturations. It also is widely used as a tocolytic drug in cases of imminent preterm labour for disruption of uterine contractions [2]. Recently it has been demonstrated that the β₂-AR agonist activity resides primarily with (R,R)-fenoterol while (S,S)-fenoterol is essentially inactive at this receptor [3]. This compound was already resolved into pairs of enantiomers on carbohydrate stationary phases. 

  Here we report new validated method for direct chromatographic resolution of (R,R) and (S,S) fenoterol diastereoisomers on  amylose tris(3,5-dimethylphenylcarbamate), offering baseline separation of this compound  (resolution factor R_S of 3.89). LOD and LOQ for the analysis were determined as 0.2 and 0.5 microgram per mililiter respectively.

1.  Anderson S. D., Rozea P. J., Dolton R., Lindsay D. A. Inhaled and oral bronchodilator therapy in exercise induced asthma. Aust. N. Z. J. Med. 5, 544-550 (1975).
2. Tejkalová H., Benesová O., Kristofíková Z., Binková B., Dlohozková N., Buresová M. The assessment of the behavioural teratogenic risk of the tocolytic drug phenoterol. Homeost. Health Dis. 33, 89-93 (1991).
3. Beigi F., Bertucci C., Zhu W., Chakir K., Wainer I. W., Xiao R. P., Abernethy D. R. Enantioselective separation and online affinity chromatographic characterization of R,R- and S,S-fenoterol. Chirality 18, 822-827 (1006).

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