Understanding of the phenomena of cell adhesion and in particular, understanding of cell adhesion on biomaterials is of crucial importance for the development of new biomaterials with excellent biocompatibility. One of the physical quantitative indexes to evaluate the quality of cell-material adhesion is its strength. Determining the strength of adhesive bonds requires applying external forces to the cells. Thus, a few methods have been developed to evaluate the strength of cell-material adhesion (micropipette, microplates, microcantilever…). These methods apply shear forces on adherent cells. The aim of our work is to propose the development of a new ultrasonic characterization method of cellular adhesion on substrates. With our method, longitudinal acoustic waves are applied on cell culture to impose a longitudinal strain on cells. Only the cells subjected to a sufficient level of strain will be detached from the substrate. The idea is to bind cell detachment rate to the longitudinal strain threshold supported by cells. From this result, we can deduce the critical force just sufficient to detach the cell. The technique is investigated for the 200 kHz ultrasound frequency. Tests were carried out with the MC3T3-E1 osteoblastic cell line on a glass substrate with different functionalizations. Our results to date provide the value of the necessary force to detach with reproducibility osteoblastic cells from glass. Other materials like dense hydroxyapatite substrates are under investigation.

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