For detection of their presence in the reaction products and to evaluate the purity of the prepared samples, a simple analytical method was developed.

High-Performance Liquid Chromatography (HPLC) is an analytical technique appropriate for the separation and determination of organic analytes in various samples, especially biological, pharmaceutical, environmental, and industrial.

Figure 1

Synthesized compounds representing structures I and II (Figure 1) were well determined and separated by reversed phase high-performance liquid chromatography in isocratic and gradient conditions. The optimization of the simultaneous determination of six compounds, shown on the Figure 1, was performed. The peaks in the chromatograms of a mixture were identified based on the retention times of isolated components injected separately. Additionally chemical structure of examined compounds was established using spectrometric methods.

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