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The use of magnetic nanoparticles as an effective separator and diagnostic of Candida albicans.

Katarzyna Niemirowicz 1Urszula Surel 1Izabela Święcicka 2Agnieszka Z. Wilczewska 3Karolina H. Markiewicz 3Alina Kułakowska 4Zbigniew Namiot 5Beata Szynaka 6Halina Car 7Robert Bucki 1,8

1. Medical University of Bialystok, Department of Microbiological and Nanobiomedical Engineering (UMB), Mickiewicza 2C, Białystok 15-222, Poland
2. University of Bialystok, Department of Microbiology, Świerkowa 20B, Białystok 15-950, Poland
3. University of Bialystok, Institute of Chemistry, Department of Natural Product Chemistry, al. Piłsudskiego 11/4, Białystok 15-433, Poland
4. Medical University of Bialystok, Department of Neurology, Białystok 15-089, Poland
5. Medical University of Bialystok, Department of Physiology, Białystok 15-222, Poland
6. Medical University of Bialystok, Department of Histology and Embryology, Białystok 15-222, Poland
7. Medical University of Bialystok, Department of Experimental Pharmacology, Białystok 15-295, Poland
8. The Faculty of Health Sciences of the Jan Kochanowski University in Kielce, Kielce 25-406, Poland

Abstract

Severe fungal infections have significantly contributed to the increasing morbidity and mortality of immunocompromised patients in need of broad-spectrum antibiotic therapy. Therefore, the increasing number of resistant fungal infections creates a constant need for the development of new therapeutic and diagnostic methods. In this study, we show that iron oxide magnetic nanoparticles and their core-shell derivatives possess high affinity to membrane of fungal cells.  Additionally, we provide evidence that these nanostructures offer an opportunity to quickly capture, eliminate, and detect presence of Candida albicans as well as to separate these pathogens from different body fluids.

This work was financially supported by the National Science Centre, Poland, Grants UMO-2012/05/N/NZ7/00534 (to KN.), UMO-2012/07/B/NZ6/03504 (to RB.)
 

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Presentation: Oral at Nano PL 2014, Symposium B, by Katarzyna Niemirowicz
See On-line Journal of Nano PL 2014

Submitted: 2014-06-28 03:42
Revised:   2014-09-26 17:37