Selol is the original Polish patented discovery. It is an organic selenium compound in oxidation state of +4. Currently Selol is a subject of intensive research that will allow to evaluate its usefulness as an anticancer compound. Alyssin is a synthetic analogue of sulforaphane  that is found in Cruciferous vegetables. Numerous studies have shown that ITCs increase cytotoxic activity of the compounds used in antitumor therapy. They are characterized by pleiotropic activity which is in many ways similar to that of Selol.

The aim of the study is to evaluate the usefulness of the MTT and CVDE in determination of the antiproliferative properties of Selol and Alyssin and their interactions.

Evaluation of antiproliferative properties of Selol, Alyssin and their combinations was conducted using two cytotoxicity assays: MTT (3 - (4,5- dimethylthiazol- 2 -yle) -2,5- diphenyltetrazolium bromide) and CVDE (crystal violet, (4 - [(4 - dimetylaminofenyl) - phenyl-methyl]-N , N -dimethyl- aniline). In MTT assay, cell viability is measured using reduction of MTT to formazan. Amount of colored, reduced MTT is proportional to the oxidative activity of cell mitochondria. While crystal violet assay is based on intercalation of the dye with the nuclear DNA. For both tests, it is assumed that the amount of product formed is directly proportional to the percentage of living cells in the population. IC₅₀ values (compound concentration at which the cell viability is 50%) were determined on the basis of assays. IC₅₀ were then used in Chou – Talalay’s interaction tests of Alyssin and Selol.

IC₅₀ values for Alyssin, determined by MTT and CVDE assay, did not differ significantly. But for Selol, IC₅₀ determined using MTT was much lower than IC₅₀ based on CVDE’s results. Interaction studies of Selol and Alyssin using the MTT method showed antagonism (weakening of the anti-proliferative effect) between the two compounds. And the results obtained by CVDE indicate different effect: additive effect of the combination of compounds.

The results indicate that the evaluation of the cytotoxic properties of the compounds may be different depending on the method used and the properties of the tested compound. Concurrently, the results of the interaction of compounds obtained using various methods may indicate different nature of the interactions taking place. When interpreting the results of antiproliferative activity of the compounds, parameter which uses a test to assess cytotoxicity should be considered.

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