Chronic long-term exposure to fluoride may leads to fluorosis. People from areas contaminated by fluoride may accumulate it predominantly in hard tissues. The analysis of the content of chemical elements in hair is an important tool in toxicological and ecological examinations and recently is the most studied biomarkers. However research works that would determine their content in black population organisms on the basis of hair analysis are not numerous. There are no studies referring to black populations living in indigenous environment, like the Mafinga region, Iringa District, in Tanzania examined by us. The aim of performed study was to analyse the content of F, Ca and Mg in easily available biological media like hair. The material was collected in October 2005 and March 2006 during anthropological examination and came from young males (n=52), students of a secondary school. Due to the broad age range of subjects (12.94-18.75), they were separated into two age groups: a younger group 16 years old (n=26) and an older group 16 years old (n=26). Hair samples were obtained from students who did not have coloured or treated hair. The concentrations of Ca and Mg were analyzed by Atomic Absorption Spectrometry. Fluoride contents were determined with a potentiometric method, using Thermo Orion ion-selective electrode (Thermo Scientific, USA). Large Ca concentration range was found for the total number of subjects (854.1 to 1290.6 µg/g d.m. with mean value for younger group 1080.48 µg/g d.m.  SD 145.30 and 1038.11 µg/g d.m.  118.75 for older group). Mean Mg content in hair for the younger group was 6.758 ± 1.895 µg/g d.m., while for older 5.848 ± 1.762 µg/g d.m. Mean F concentration for the older group of subjects was 103.563 ± 79.501 µg/g d.m., being significantly higher than in younger group: 53.009 ± 29.339 µg/g d.m. (p=0.0055). Fluoride concentration in hair was positively correlated with age (Rs=0.38, p=0.008). Body mass index (BMI = kg/m2) was calculated for the subjects showing that out of 52 men participating in the study 9.6% was characterised by mediocre nutrition (BMI ≤18.49). No statistically significant correlations were found between BMI and the analysed chemical elements. The analysis of the content of trace elements in hair of the examined young males showed deficiencies in Mg and low concentration of fluoride.

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