OBJECTIVE: The periparturient period and delivery may be effected on oxidative stress for pregnant female and newborn mammalian infants. In this period a newborns is exposed to the loss of balance between the production of reactive oxygen species and the capability of mechanisms protecting against the oxidation effects.

Glutathione (L--glutamyl-L-cysteinylglycine), which is present in all mammalian tissues, provides reducing capacity for several reactions and plays an important role in detoxification of hydrogen peroxide, other peroxides, free radicals and is a key factor in intracellular antioxidant defense mechanism. We postulated that it might modulate the adaptational response of the newborn to a higher oxygen concentration after birth. The aim of this study was to evaluate glutathione metabolism during the first week of life in goat kids (Capra hircus).

MATERIALS AND METHODS: The study was performed on 12 goat kids in the first week of life. The animals were kept in coops together with their mothers, were fed with colostrum and mother’s milk; they had free access to fodder (hay, straw) and water.

In whole blood we determined total GSH (tGSH), GSH and GSH disulfide (GSSG) concentrations, GSH/GSSG ratio (colorimetric method; Bioxytech GSH/GSSG-412 Assay) and the activites of the erythrocyte GSH reductase – GR (Bioxytech GR-340 Assay).

RESULTS: The contents of total GSH in whole blood in newborn goat kids ranged from 681,32 µM to 965,78 µM (782,52 ± 134,80 µM). We observed relatively high GSH/GSSG ratio (155,68 ± 0,64) and erythrocyte GSH concentration (772, 60 ± 133,11 µM); the concentration of GSH disulfide was low (4,96 ± 0,85 µM). Erythrocyte GSH reductase activity was low (3,65 ± 0,87 U/g Hb). In the presented study significant correlations were found between GR and tGSH, GSSG, GSH.

CONCLUSION: Relatively low concentration of GSSG in blood of goat kids and high GSH/GSSG ratio indicate on high metabolic capacity the glutatione antioxidant system in newborn goat kids in first week of life, though low erythrocyte GSH reductase activity.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/10827 must be provided.

1. The effect of the periparturient period on plasma fluoride concentrations in goats (Capra hircus)
2. The content of fluoride, calcium and magnesium in the hair of young men of the Bantu language group from Tanzania versus environmental and social conditioning
3. Fluoride in the brain and pineal gland of two duck species Aythya fuligula and Melanitta fusca wintering in northwestern Poland
4. Effect of supplementation with conjugated dienes of linoleic acid (CLA), the main components of the preparations for slimming, on the fluoride, calcium and magnesium content in the hard tissues and serum of mice.
5. Fluoride influence on energy synthesis in skeletal muscle cell line CCL136.
6. Fluoride and lead influence on apoptosis processes in liver cell line HepG2.
7. Effect of Sodium Fluoride on Catalase Activity in THP-1 Macrophages
8. Simultaneous Pb and fluoride exposure can influence cyclooxygenase and lipoxygenase activity acting as a pro-infammatory factor in differentiated human THP1 monocytic cells
9. Inhibition of erythrocyte phosphoribosyltransferases (APRT and HGPRT) and decreased energy charge value in rats chronically exposed to lead
10. The influence of the soya isoflavones received pre- and postnatally on the mineral metabolism and the function of the male reproductive system in rats
11. Changes in antioxidant status and energy metabolism of erythrocytes in experimental diabetes in rats
12. Paracetamol metabolism in black-and-white and black-and-white x holstein-friesian cross breed calves